侵袭性垂体腺瘤与非侵袭性垂体腺瘤的基因差异表达谱研究.docVIP

侵袭性垂体腺瘤与非侵袭性垂体腺瘤的基因差异表达谱研张铃铛※ 霍钢※※肖文峰△ 杜安东※ 吴留洋※重庆医科大学附属第一医院神经外科 重庆 400016）摘要】目的 利用基因芯片研究侵袭性垂体腺瘤与非侵袭性垂体腺瘤的基因差异表达谱方法 应用Illumina人全基因组基因表达芯片检测6例侵袭性垂体腺瘤以及6例非侵袭性垂体腺瘤组织的基因表达谱，并选择其中差异表达的两个基因进行逆转录一聚合酶链反应(reverse transcription polymerase chain reaction，RT-PCR)验证。结果 基因芯片筛选出差异表达基因153条，包括63条上调表达基因和90条下调表达基因。对上调基因CDH12(N-cadherin 2）和下调基因KLF4 (Kruppel-like factor 4)行RT-PCR验证结果与芯片结果相符。结论 侵袭性垂体腺瘤与非侵袭性垂体腺瘤的基因表达谱存在差异，其中差异表达基因与KLF4可能通过作用于细胞在垂体腺瘤的侵袭性中发挥着一定作用。关键词】垂体腺瘤 基因芯片 基因表达谱 侵袭性ifferential gene expression profiles in invasive pituitary adenomas and noninvasive pituitary adenomas Zhang Lingdang，Huo Gang，Xiao Wenfeng，Du Andong，Wu Liuyang，Che Pengyu. Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University,youyi road No.1, Chongqing 400016 ,China. Tel [Abstract] Objective: Use gene microarray to study differential gene expression profiles in invasive pituitary adenomas and noninvasive pituitary adenomas. Methods: Application the Illumina gene expression in human whole genome microarray detect gene expression profiles in 6 cases of invasive pituitary adenomas and 6 cases of non-invasive pituitary adenomas, analysis of differentially expressed genes, and choose two differentially expressed genes validated by Reverse transcription polymerase chain reaction (reverse transcription polymerase chain reaction, RT-PCR). Results: Gene microarray selected 153 differentially expressed genes, including 63 up-regulated genes and 90 down-regulated genes，Some of differentially expressed genes of cell adhesion, oncogenes, signal transduction related genes. On the up-regulated genes CDH12 (N-cadherin 2), and down-regulated genes KLF4 (Kruppel-like factor 4) line RT-PCR validation results was consistent with the microarray. Conclusion :Gene expression profiles of invasive pituitary adenomas are different from non-invasive pituitary adenomas, which KLF4 differentially expressed genes SERPINE1 and the role of cell physiological processes may be throug