电离-飞行时间-质谱技术分析阿霉素肾病大鼠尿液-第三军医大学学报.DOCVIP

SELDI-TOF-MS筛选微小病变型肾病综合征尿液生物标志物 林 青1，郑 健2，陈蓉艳1，艾 斯2 （350004福建 福州福建中医学院附属人民医院1检验科，2儿科） surface enhanced laser desorption/ionization time of flight-mass spectrometry ，SELDI-TOF-MS）进行微小病变型肾病综合征（minimal-changed nephrotic syndrome，MCNS）大鼠尿液生物标志物筛选的意义。方法 SD大鼠30只随机分为正常对照组、模型对照组和强的松治疗组。除正常对照组外，其余大鼠经尾静脉一次性注射阿霉素5.5 mg/kg复制MCNS模型，造模10 d后分别予强的松灌胃MCNS大鼠尿液生物标志物的筛选提供一个可行的检测途径。所获得的差异性蛋白峰对MCNS的早期诊断、治疗监测和寻找药物治疗的靶目标具有重要的意义。 [关键词]SELDI-TOF-MS；微小病变型肾病综合征；尿液；生物标志物；质控 [中图法分类号] [文献标志码]A SELDI-TOF-MS as a method for biomarker discovery in the urine of minimal-changed nephrotic syndrome LIN Qing1, ZHENG Jian2, CHEN Rong-yan1, AI Si2 (1Department of Clinical Laboratory, 2Department of Paediatrics, The Affiliated People’s Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou 350004,China) [Abstract] Objective To study the use of optimal surface enhanced laser desorption/ionization time of flight-mass spectrometry (SELDI-TOF-MS) for detecting biomarkers in the urine of minimal-changed nephrotic syndrome(MCNS). Methods Thirty SD rats were randomly divided into normal control group, untreated group and prednisone group. Except the normal control group, rats were injected once via caudal vein with adriamycin ( 5.5 mg/kg ) to induce MCNS. 10 days later, the rats were administered with prednisone or distilled water for 21d , respetcively. Collecting the urine of rats, urinary peptide or protein peaks were detected by SELDI-TOF-MS and weak cation exchange proteinchip CM10. The differentially expressed protein and polypeptides were analyzed with Biomarker Wizard Software. Results Under optimal experimental conditions, the average variation coefficients of the relative peak intensity in control were 18.0 %（between 7.50 % and 35.89 %）. In the different range of m/z values , there were 33 significantly different mean peak intensities in the untreated group as compared with normal control group （P0.05）, 21 of them were up expressed , 12 of them were down