论着Smad7在氯化锂-匹鲁卡品诱发癫痫大鼠脑组织中的表达研究刘.DOCVIP

论著 Smad7Smad7在氯化锂-匹鲁卡品诱发癫痫大鼠中不同病程的表达情况，探讨Smad7在癫痫形成中的作用机制。方法 将60只健康雄性SD大鼠随机分为六组：正常对照组（n=10）和经氯化锂-匹鲁卡品诱发癫痫后的五个实验组，分别是24 h（n=10），72 h（n=10），1w（n=10），1m（n=10），2m（n=10）。使用Western blot和免疫组化检测Smad7在氯化锂-匹鲁卡品诱发癫痫大鼠海马及颞叶皮质的表达情况。结果 与正常对照组相比，Smad7在氯化锂-匹鲁卡品诱发癫痫大鼠的表达显著降低（P0.01）。结论 Smad7表达降低可能参与癫痫形成，其机制主要是通过调控TGF-β信号通路来实现。 [关键词] Smad7；癫痫；TGF-β信号通路 [基金项目] 国家自然科学基金；重庆市教育委员会科研项目（KJ120302） [通讯作者] 余维华，E-mail：weihuayu0707@Expression pattern of Smad7 in the brain of lithium chloride-pilocarpine induced epilepsy rats Liu Wei（Neuroscience research centre, Department of Human Anatomy, Chongqing Medical University, Chongqing , 400016, China） [Abstract] Objective To observe the expression of Smad7 in lithium chloride-pilocarpine induced epilepsy rats in different course of the disease, and hope to understand how Smad7 function in the mechanism of epilepsy. Methods Sixty healthy adult male Sprague-Dawley rats were randomly divided into six groups: normal control group (n =10) and five experimental subgroups respectively 24 h (n = 10), 72 h (n = 10), 1w (n =10), 1m (n = 10), and 2m (n = 10) after SE induced by lithium chloride–pilocarpine. We examied the expression of Smad7 in the hippocampus and temporal lobe cortex of lithium chloride-pilocarpine induced epilepsy rats through using western blotting and immunohistochemistry. Results Both western blotting and immunohistochemistry showed that Smad7 was significantly decreased in the lithium chloride -pilocarpine induced epileptic rats compared with the normal rats（P0.01）. Conclusion Our results indicate that the down regulation of Smad7 might be involved in epileptogenesis, and the mechanism mainly through regulating the TGF-β signaling pathways. [Key words] Smad7; epilepsy; TGF-β signaling pathways Supported by the National Natural Science Foundation of China ; the Scientific Research Project of Chongqing Municipal Education Commission (KJ120302). Corresponding author: Yu Weihua, E-mail：weihuayu0707@TGF-β信号通路参与癫痫发生。研究发现在