鸟类组织稳定同位素分析样品的预处理方法-动物学杂志.pdfVIP

动物学杂志 Chinese Journal of Zoology 2016, 51(3): 477 ~ 486 鸟类组织稳定同位素分析样品的预处理方法 ①② ② ①* 李继荣 李来兴 杨 乐 ① 西藏自治区高原生物研究所 拉萨 850000；② 中国科学院西北高原生物研究所 中国科学院高原生物适应与进化重点实验室 西宁 810001 摘要：稳定同位素分析样品在进行同位素质谱分析之前需要适当的处理，然而如何处理尚未形成统一 的标准。本文结合鸟类组织稳定同位素分析样品的测试流程，介绍了鸟类学研究领域稳定同位素技术 应用中涉及的分析样品解冻清洗、干燥、分离纯化、研磨和储存等前处理方法，并对不同组织常用的 处理方法及分析样品测定中有待解决的问题展开了讨论。旨在抛砖引玉，为利用稳定同位素技术开展 鸟类学方面的研究提供科学参考和技术支持。 关键词：稳定同位素；鸟类；组织；前处理 中图分类号：Q955 文献标识码：A 文章编号：0250-3263 （2016 ）03-477-10 Pretreatment Methods for Stable Isotope Analysis of Avian Tissues ①② ② ①* LI Ji-Rong LI Lai-Xing YANG Le ①Tibet Plateau Institute of Biology, Lhasa 850000; ②Key Laboratory of Adaptive and Evolution of Plateau Bio logy, Northwest Plateau Institute of Biology , Chinese Academy of Science, Xining 810001, China Abstract: Stable isotope analysis samples require proper treatment before isotope mass spectrometry analysis, but there is still no standard method for sample pretreatment. Combining with the stable isotope analysis testing process of avian tissues, this paper introduces the sample pretreatment methods, such as thawing and cleaning, drying, isolation and purification, grinding and storage, which are useful for isotope technology applications in Ornithology. Different tissues pretreatment methods and problems in sample analysis are also discussed. We hope this review will provide some scientific references and technical supports for the application of stable isotope technique in Ornithology. Key words: Stable isotope; Avian; Tissues; Pretreatmen