SMC3对肺癌A549细胞增殖_迁移和成球能力的影响_余冬梅.pdfVIP

网络出版时间：2017-09-15 11:32:35 网络出版地址：/kcms/detail/31.2035.Q1132.004.html 中国细胞生物学学报 Chinese Journal of Cell Biology 2017, 39(10): DOI: 10.11844/cj cb.2017.10.0095 SMC3对肺癌A549细胞增殖、迁移和成球能力的影响 * * 余冬梅 黄 颖 安 输 杨 洋 刘 莹 郝 倩 徐天瑞 郭晓汐 ( 昆明理工大学生命科学与技术学院细胞信号传导实验室, 昆明650500) 摘要 该文通过RNA干扰方法下调染色体结构维持蛋白3(structural maintenance of chromo- somes protein 3, SMC3)的表达, 探究其对人肺癌细胞A549的影响。用Western blot验证SMC3敲低 的效果, 用CCK-8实验、Transwell 实验检测SMC3敲低对A549细胞的增殖和迁移能力的影响, 用细 胞成球等实验检测SMC3敲低对A549细胞干性的影响。结果显示, 该研究成功地构建了pSUPER- SMC3干扰质粒, 将其转染A549细胞后SMC3蛋白质水平明显降低。实验组(pSUPER-SMC3)与空 白组(control)及阴性对照组(pSUPER)相比, 细胞增殖和迁移能力明显下降(P0.01), 细胞成球数减 少, 干性减弱。该实验结果表明,下调SMC3蛋白质水平可以抑制A549细胞的增殖和迁移能力, 减弱 细胞干性, 提示SMC3对肺癌的发生和发展可能具有促进作用。该实验为寻找潜在的抗肺癌方法 提供了新的实验依据。 关键词 RNA干扰; A549细胞; SMC3; 增殖; 迁移 Effects of SMC3 on the Proliferation, Migration and Sphere Formation of Lung Cancer A549 cells Yu Dongmei, Huang Ying, An Shu, Yang Yang, Liu Ying, Hao Qian, Xu Tianrui*, Guo Xiaoxi* (Cell Signaling Lab, Kunming University of Science and Technology, Kunming 650500, China) Abstract In this study, the level of SMC3 protein was down-regulated by RNA interference, then we explore the effect of SMC3 on human lung cancer cell line A549. The level of SMC3 protein was determined by Western blot. The effect of SMC3 down-regulation on the proliferation and migration of A549 cells was detected by CCK-8 assay and Transwell assay. The effect of SMC3 down-reg