猪链球菌2型荚膜基因pcr快速检测方法的建立及试剂盒的研制.pdfVIP

生物技术通报 # # BIOTECHN OLOG Y B ULLETIN 2006 2 PCR 姜焱 张敬友 王凯民 唐泰山 陈国强 张常印 (, 210001) : 根据猪链球菌 2型的荚膜基因 2H 序列设 一对引物, 成功地扩增了荚膜基因, 并建立了检测猪链 球菌 2型的 PCR 方法用 ScaI进行确认, 获得了与预期大小一致的 389bp和 300bp的两 个片段然后对该方法 的敏感性特异性进行了研究结果表明, 敏感程度可达 10 个细菌; 对其他细菌 PCR 检测结果均呈阴性, 表明建 立的猪链球菌 2型荚膜基因的 PCR检测方法, 其特异性和敏感性均很高, 可作为猪链球菌病快速诊断的方法在 建立 PCR 方法的基础上, 研制成试剂盒, 并对试剂盒的特异性敏感性和稳定性进行了研究 : 猪链球菌 2 型 荚膜基因 PCR 试剂盒 Construction a PCR for Rapid Detecting CPS 2H ofSw ine Strep tococcus SuisType 2and Development aKit Jiang Y an Zhang Jingyo W ang Kami in Tang Taishan Chen G oqiang Zhang Changyin (J iang su E ntry-Ex it lnsp ection and Quarant ine B urea u, N anj ing 210001) Abstract: Accord ing to the GenBank open seq ence of CPS 2H of sw ine S trep tococcus su is Type 2, a pair of prmi er w as designed in order to am plify CPS 2H gene and constr ct a PCR to detect sw ine S rrep tococcu s suis Type 2. The PCR pro- d ction w as digested by Scal. Therew ere two fragm ents, 389bp and 300bp, respectively. Then the sens itivity and specificity w ere st died. The res lt dem onstrated the PCR sensitivity co ld detect 10 bacteria, and the other bacteria was negative re- s lt. The PCR had high sensitivity and specificity and co ld be sed to detect sw ine Strep tococcus suis Type2. On the basis of PCR m ethod, the k it was developed and the k it of specificity, sensitivity and stabk lity w ere st d ied Key w ords: Sw ine streptococ s s is type2 CPS PCR K it 2 2 2 , , R , , 2 , , , , ,