多效唑对青钱柳苗木生理特性的影响南京林业大学.docVIP

多效唑对青钱柳生理特性的影响郭楠张颖颖刘娜娜谢寅峰摘 要以3年生青钱柳(Cyclocarya paliurus)苗木为材料，采用叶面喷施的方法探讨不同浓度多效唑(PP333)对青钱柳生理特性的影响。结果表明：适当浓度的多效唑处理能有效促进苗木地茎相对生长量，提高叶片可溶性蛋白、可溶性糖以及药用次生代谢物三萜、山奈酚和槲皮素的含量，刺激超氧化物歧化酶(SOD)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)活性的升高，但对苗高生长起抑制作用。400mg/l浓度处理对地茎相对生长量、可溶性蛋白和槲皮素含量促进作用最佳，与对照相比，分别增加180.49%、48.14%和100.0%，均达显著差异（P0.05）。600mg/l浓度处理对可溶性糖、三萜、山奈酚含量以及SOD、POD、PAL活性刺激作用最强，分别比对照增加32.22%、46.02%、22.88%、46.66%、53.68%和67.51%，均差异显著。以上结果说明，适当浓度的多效唑处理可调节青钱柳苗木代谢特性，提高苗木抗逆性，刺激药用成分的合成。研究结果为青钱柳苗木培育及其次生代谢物含量调控的研究提供依据。 [关键词]青钱柳；多效唑；生理特性；次生代谢物 Effects of PP333 on the Physiological Characteristics in Cyclocarya paliurus Seedlings GUO Nan ZHANG Ying-ying LIU Na-na XIE Yin-feng Abstract：The effects of aclobutrazol (PP333) on the physiological characteristics in Cyclocarya paliurus leaves of 3-year-old seedlings were investigated by spraying different concentrations of PP333. The results showed that the stems relative growth yield, content of soluble protein, soluble sugar as well as the drug secondary metabolites including triterpenoid, kaempferol, and quercetin in leaves of Cyclocarya paliurus were effectively improved by adding a suitable concentration of PP333, the activity of superoxide dismutase (SOD), peroxidase(POD) and phenylalanine ammonia-lyase(PAL) increased at the high levels, while the seedings relative growth yield were delayed. 400mg/l concentration treatment was the best to promote the stems relative growth yield, content of soluble protein and quercetin. Compared with the control, the above indexes increased by 180.49%、48.14% and 1% respectively. 600mg/l concentration treatment improved the content of soluble sugar, triterpenoid and kaempferol as well as the the activity of SOD, POD and PAL with a maximum stimulation. Compared with the control, the above indexes increased by 32.22%、46.02%、22.88%、46.66%、53.68% and 67.51%, all results remarkable. The results suggested that suitable concentration of PP333 regulates the metabolic characteristic of Cyclocarya paliurus, increasing seedling resi