甘蔗健康种苗繁殖培养基正交试验分析-甘蔗糖业.pdfVIP

甘蔗糖业 2011 年第6 期，2011 年12 月 Sugarcane and Canesugar No. 6, Dec. 2011 甘蔗健康种苗繁殖培养基正交试验分析 樊丽娜，何慧怡，陈月桂，罗青文，劳方业，谭嘉娜，邓海华，杨俊贤，李奇伟，齐永文 (广州甘蔗糖业研究所 广东省甘蔗改良与生物炼制重点实验室，广东广州510316) 摘 要：本研究选取 ROC22 、粤糖 00-236 2 个甘蔗品种为实验材料，通过正交试验设计，研究 6-苄氨 基嘌呤(6-BA) 、噻苯隆(TDZ) 、萘乙酸(NAA) 、多效唑(PP333)4 种植物生长调节剂对甘蔗健康种苗繁殖 过程中丛芽苗分化的影响。实验结果表明，甘蔗健康种苗丛芽苗的分化主要受各品种遗传特性决定， 不同品种对繁殖培养基的激素水平要求差异较大，在 4 种激素中, 对 ROC22 丛芽苗分化影响的主次因 素为 TDZ ＞NAA ＞PP333 ＞6-BA ，对粤糖 00-236 丛芽苗分化的影响主次顺序为 6-BA ＞TDZ ＞NAA ＞ PP333 。实验结果表明，适宜的激素浓度组合是提高繁殖效率的关键，因此，甘蔗健康种苗繁殖过程中， 应根据不同品种生长特性筛选最优培养基。 关键词：甘蔗；健康种苗；繁殖培养基 中图分类号：S566.1 文献标识码：A 文章编号：1005-9695(2011)06-0006-05 Analysis of Propagation Medium of Sugarcane Healthy Plants by Orthogonal Experiment Design FAN Li-na, HE Hui-yi, CHEN Yue-gui, LUO Qing-wen, LAO Fang-ye, TAN Jia-na, DENG Hai-hua, YANG Jun-xian, LI Qi-wei, QI Yong-wen (Guangzhou Sugarcane Industry Research Institute/Guangdong Key Lab of Sugarcane Improvement Biorefinery, Guangzhou, 510316) Abstract: In this study, the effect of 6-BA, NAA, PP333, TDZ in medium for the propagation of healthy plants of Yuetang00-236, ROC22 was investigated by using orthogonal experiment design. The results showed that the best medium for the three varieties were different. Among the four plant growth regulators, the most important factor for the propagation of Yuetang 00-236 was 6-BA, followed by TDZ, NAA and PP333, but for that of ROC22, the important factor was TDZ, followed by NAA, PP333 and 6-BA. The study indicated that the combination of the concentration of the growth regulators is the key factor for the sugarcane healthy plant propagating efficiency, and therefore, special m