甜椒花药培养及再生植株技术体系的研究-热带亚热带植物学报.pdfVIP

热带亚热带植物学报 2016, 24(3): 296 ~ 301 Journal of Tropical and Subtropical Botany 甜椒花药培养及再生植株技术体系的研究 张天翔，林宗铿，蔡坤秀，杨俊杰，曹明华 (福建省热带作物科学研究所，福建 漳州 363000) 摘要：为建立甜椒(Capsicum annuum var. grossum)花药培养及再生植株技术体系，对影响花药胚状体诱导和分化的因素进行 了研究。结果表明，培养基组成对胚状体诱导率的影响以NAA ＞基本培养基＞椰乳＞KT ，最佳胚状体诱导培养基为NTH + 0.1 mg L–1 NAA + 10%椰乳 + 1 mg L–1 KT + 50 μmol L–1 AgNO3 + 30 g L–1 蔗糖 + 5 g L–1 琼脂 + 2 g L–1 活性炭。花药经过 24 h 低温预处理和 8 d 高温预培养后，胚状体诱导率可达 23.38% 。植物生长调节剂对胚状体出芽率的影响为 6-BANAAIAA, 最佳胚状体分化培养基为 NTH + 1 mg L–1 6-BA + 0.3 mg L–1 NAA + 0.1 mg L–1 IAA + 30 g L–1 蔗糖 + 5 g L–1 琼脂。胚芽转入 –1 –1 –1 1/2MS + 0.5 mg L IBA + 30 g L 蔗糖 + 5 g L 琼脂培养基后，生根率可达 92.5% 。 关键词：甜椒；花药培养；胚状体；再生植株 doi: 10.11926/j.issn.1005-3395.2016.03.008 In vitro Culture and Plant Regeneration Technology System of Capsicum annuum var. grossum Anthers ZHANG Tian-xiang, LIN Zong-ken, CAI Kun-xiu, YANG Jun-jie, CAO Ming-hua (Fujian Institute of Tropical Crops , Zhangzhou 363000, Fujian, China) Abstract: The aim was to establish plant regeneration system from anthers of Capsicum annuum var. grossum . The influence factors on embryo induction and differentiation were studied. The results showed that the influences of medium compositions on embryo induction were in the order of NAA ＞basal medium ＞coconut milk ＞KT. –1 –1 The optimum medium for embryo induction was NTH + 0.1 mg L NAA + 10% coconut milk + 1 mg L KT + –1 –1 –1 –1 50 μmol L AgNO3 + 30 g L sugar + 5 g L agar + 2 g L activated carbon. After pretreated for 24 h in low temperature and precultured for 8 d at high temperature, embryo induction rate could reach 23.38%. The effect of plant growth regulator on bud rate from embryo was in the order of 6-BANAAIAA, and the optimum medium