药用植物裸花紫珠的组织培养与快速繁殖研究.PDFVIP

中国农学通报 2013,29(7): 127-132 Chinese Agricultural Science Bulletin 药用植物裸花紫珠的组织培养与快速繁殖研究 潘 梅，黄 赛，王景飞，吕德任，戚华莎 （海南省农业科学院园林花卉研究所，海口571100） 摘 要：以种子为外植体，MS 为基本培养基，通过比较不同植物生长调节剂种类和浓度配比、培养条件以 及生根苗的移栽基质，建立裸花紫珠组织培养快速繁殖体系。结果表明：外植体表面消毒以75%酒精预 处理10 s ，再用0.1% HgCl 浸泡10 min ，效果最好；种子在MS 基本培养基上萌发；丛生芽继代增殖的最 2 适温度为28℃，最适光照强度为1500 lx ；培养基MS+ 6-BA 2.00 mg/L+ NAA 0.05 mg/L 适宜继代增殖，30 天的增殖系数为10.87；培养基MS+ NAA 0.50~0.75 mg/L 适宜诱导生根，生根率100% ；生根苗移栽于河 沙、珍珠岩和表土(1:1:1)的混合基质中，成活率96% ，高生长量最大。运用该组培快繁技术，可以实现裸 花紫珠的工厂化育苗。 关键词：裸花紫珠；种子；丛生芽；组织培养；快速繁殖 中图分类号：Q943.1 文献标志码：A 论文编号：2012-3159 Tissue Culture and Rapid Propagation of Callicarpa nudiflora PanMei,HuangSai,WangJingfei,LvDeren,QiHuasha (GardenFlowerResearchInstituteofHainanAcademyofAgriculturalSciences,Haikou571100) Abstract:Usingseedsasexplant,MSasbasicculturemedium,thetechniquesoftissuecultureandrapid propagationof Callicarpa nudiflora werestudiedbycomparingwithtypesandconcentrationsofplantgrowth regulators,cultureconditions,typesoftransplantingmedium.TheresultsshowedthatintheMSbasicmedium seedgerminationbemade;themostsuitableprocedureforsterilizationwastosoakexplantsinto0.1%HgCl2 solutionfor 10minafterpretreatmentwith75%alcoholfor 10s.Suitabletemperatureofbudssubcultureand proliferationwas 28℃,theoptimumlightintensitywas 1500lx.MS+ 6-BA 2.00 mg/L+NAA 0.05 mg/Lbe suitableforsubcultureandproliferation,thepropagationcoefficientwas 10.87 every 30 days;themost optimumrootingmediumwasMS+NAA0.50-0.75mg/L,andtherootingratewas 100%.Therootingseedling wereeffectivelytransplantedinriversand,perliteandtopsoil(1:1:1),thehighestsurvivalratewas 96%,and thelargestamountofgrowth.Usingthetechnologyoftissuecultureandrapidpropagationof Callicarpa nudifl