表观遗传药物对体细胞核移植胚胎发育的促进作用-中国细胞生物学学报.pdfVIP

网络出版时间：2017-10-25 17:44:38 网络出版地址：/kcms/detail/31.2035.Q1744.022.html 中国细胞生物学学报 Chinese Journal of Cell Biology 2017, 39(11): DOI: 10.11844/cj cb.2017.11.0147 表观遗传药物对体细胞核移植胚胎发育的促进作用 * * 马盼盼 杨树宝 栾维民 马 馨 (吉林农业大学动物科学技术学院, 长春130118) 摘要 体细胞核移植(somatic cell nuclear transfer, SCNT)是利用卵母细胞胞质中的重编程物 质对高度分化体细胞核进行重编程作用使其恢复全能性并发育为新个体的技术。在SCNT过程中, 表观遗传修饰参与卵母细胞的重编程, 如DNA 甲基化修饰和组蛋白的翻译后修饰。这些重编程的 异常修饰会对SCNT胚胎的发育产生不良影响。表观遗传药物, 如DNA 甲基转移酶抑制剂和组蛋 白去乙酰基酶抑制剂, 可改善表观遗传修饰的异常现象, 促进体细胞核移植重构胚的重编程。该文 对SCNT胚胎重编程过程中的异常表观遗传修饰以及近年来报道的表观遗传相关药物进行综述, 并 进一步探讨了这些药物对SCNT胚胎发育的促进作用。 关键词 表观遗传药物; 体细胞核移植; 重编程; 胚胎 Progress on Epigenetic Drugs in Development of Somatic Cell Nuclear Transfer Embryos Ma Panpan, Yang Shubao, Luan Weimin*, Ma Xin* (College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China) Abstract Somatic cell nuclear transfer (SCNT) is a technology that makes use of cytoplasmic reprogramming material in the oocyte to make reprogramming of highly differentiated somatic cell nuclear to restore the totipotency and develop into a new number. In SCNT, epigenetic modifications participate in reprogramming of oocyte, such as modifications of DNA methylation patterns and histone modification after translation, their abnormal expression has adverse impacts on the development of SCNT embryos. Epigenetic drugs, such as DNA methyltransferase inhibitors and histone deacetylase inhibitors, can improve t