福建胜红蓟黄脉病病原的分子鉴定-中国科技论文在线.pdfVIP

中国科技论文在线 福建省胜红蓟黄脉病病原的分子鉴定 # 陈家盛，胡汝检，朱重庆，林文武，林文忠，林丹，张洁** （福建农林大学植物病毒研究所/福建省植物病毒学重点实验室） 5 摘要：双生病毒是一类近年来对我国农作物产生危害逐渐加重的病毒。本研究对福建农林大 学校内表现黄花叶、明脉等疑似感染双生病毒症状的胜红蓟进行病原鉴定。利用双生病毒 特异性简并引物PA/PB 扩增得到500 bp 的DNA-A 部分片段，再根据已获得片段设计特异 性引物将其全长剩余部分约2.3 kb 扩增出来。序列拼接得到全长2734 nts 。序列分析发现， 该病毒与台湾一种胜红蓟黄脉病毒的同源性高达99.5% 。同时，利用引物β01/β02 扩增出了 10 DNA-β 卫星的全长 （1346 nts），序列分析发现，卫星分子也与一种胜红蓟黄脉病毒伴随的 卫星分子的同源性最高，达 98.1% 。因此，判断此种病毒为胜红蓟黄脉病毒的一种分离物。 关键词：植物病毒；双生病毒；胜红蓟；DNA-A ；DNA-β 中图分类号：S432.4+1 15 Molecular Identification of the Pathogen inducing Ageratum Yellow Vein Disease in Fujian CHEN Jiasheng, HU Rujian, ZHU Chongqing, LIN Wenwu, LIN Wenzhong, LIN Dan, ZHANG Jie (Institute of Plant Virology, Fujian Agriculture and Forestry University/ Key Laboratory of Plant 20 Virology of Fujian Province) Abstract : Geminiviruses have caused a significantly increasing damages to crops in our country in recent years. In this study, Ageratum samples showing obvious yellow mosaic leaves and vein clearing geminiviral-like symptoms were selected in campus of Fujian Agriculture and Forestry University. By using degenerate primers PA/PB for geminivirus, a partial DNA-A sequence with 500 bp was amplified. 25 Then, using designed specific primers, the left sequence (around 2.3 kb) of the full-length was obtained. Sequence splicing showed the full length of 2734 nts. Sequence analysis showed that this virus had a high sequence identity of 99.5% with Ageratum yellow vein virus isolated from Taiwan. Meanwhile, using primersβ01/β02 for the betasatellite, full-length of DNA-β w