定量RT-PCR检测雌二醇诱导的青鳉鱼基因表达-北京大学城与环境.PDF

中国环境科学 2006,26(5) ：599~602 China Environmental Science 定量 RT-PCR 检测雌二醇诱导的青鳉鱼基因表达 1,2 1 1* 2 侯彦峰 ,张照斌 ,胡建英 ,范光丽 (1.北京大学环境学院,北京 100871 ；2.西北农林科技大学动物科技学院, 陕西 杨凌 712100) 摘要：采用实时定量RT-PCR(Q-RT-PCR)方法,对暴露60d雌二醇的雄性青鳉鱼肝脏内卵黄蛋白原(VTG)和卵壳前体蛋白(CHG)基因表达进 行研究.结果表明,0.1, 1.0, 10.0, 100.0ng/L的雌二醇使肝脏内VTG-I,VTG-II,CHG-L和CHG-H基因表达被显著诱导.其中VTG-I显示出较好的 剂量—效应关系,是较为理想的生物标记物基因.在0.1ng/L雌二醇暴露组中,VTG-I和VTG-II 的表达量均显著高于对照组(P0.05),表明本方 法具有检测环境中低剂量雌二醇暴露的潜力. 关键词：雌二醇；青鳉鱼；生物标志物；实时定量RT-PCR ；卵黄蛋白原；卵壳前体蛋白 中图分类号：R994.6 文献标识码：A 文章编号：1000-6923(2006)05-0599-04 Gene expression in liver of male medaka (Oryzias latipes) induced by estradiol of low concentration examined, 1,2 1 1* 2 survived with real-time quantitative RT-PCR. HOU Yan-feng , ZHANG Zhao-bin , HU Jian-ying , FAN Guang-li (1.College of Environmental Science, Peking University, Beijing 100871, China ；2.College of Animal Science and Technology, Northwesten Agriculture and Forestry University, Yangling 712100, China). China Environmental Science, 2006,26(5) ：599~602 Abstract ：The studies on the gene expression of vitellogenin protein origin and choriogenin in the liver of male medaka exposed estradiol for 60 days by real-time quantitative RT-PCR(Q-RT-PCR) technique indicated that the VTG-I, VTG-II, CHG-L and CHG-H, gene expression in liver was induced markedly by estradiol of 0.1, 1.0, 10.0 and 100.0ng/L, in which VTG-I showed a better dose response relation, which was more theoretical biomarker gene. In the exposed group of 0.1ng/L estradiol, the expression amount of VTG-I and VTG-II were higher markedly than those of the control group (P 0.05), indicating that this technique possessed the potential to examine, survey the low dose estradiol expos