血液干细胞之临床应用.pdfVIP

血液幹細胞之臨床應用 與幹細胞庫之管理 中國醫藥大學附設醫院 醫學研究部 林嬌玲 102.06.15 Characteristics of Hematopoietic Stem Cell • Multi-lineage differentiation (white HSC cells, red cells, platelet) Self Self • Self-renewal capacity Renewal Renewal differentiation • Ability to reconstitute myeloablated patient HPC differentiation Hoffbrand AV, Pettit JE. Clinical Hematology, 2nd ed. Mosby-Wolfe The percentage of CD34+ cell in different source • 1-3 % of nucleated cell fraction in bone marrow (BMT:1960 年) • 0.1-0.2 % in peripheral blood (PBSCT:1980 年) ： GCSF-mobilization • 0.8-1.2 % in cord blood (CBSCT:1989 年) (Yin et al., 1997) History 1914: Alexis Carrel: cells or organs from another one would always be recognized as foreign and then be rejected. 1951: Lorenz E. et.al.: Radiation protection could be conferred by bone marrow infusion. (due to factor in bone marrow will stimulate marrow recovery). 1957: Thomas ED et.al.: 1st attempt of BMT in human beings （failure; HLA was identified in 1960+ ） 1959: Thomas ED et.al.: 2nd attempt of BMT in identical twin （hematological recovery occurred in 2 weeks, but leukemia recurred months later ） Type of HSCT Donor Selection Procedure Allogeneic:MSD / MUD BMT 兄弟姊妹 / 非親屬 Syngeneic同卵雙胞胎: PBSCT Autologous