家蝇幼虫蝇油的提取及其抗衰老作用研究.pdfVIP

目 录 摘要I 1 前言1 2 仪器、试剂与材料2 2.1 仪器2 2.2 试剂2 2.3 材料2 3 实验方法3 3.1 蝇油的提取3 3.2 蝇油理化常数的测定4 3.3 药用软膏的制备5 3.4 蝇油软膏的抗衰老研究6 3.5 蝇油的抑菌实验7 4 结果与分析8 4.1 家蝇幼虫蝇油的提取方法8 4.2 工艺验证试验9 4.3 蝇油理化性质的测定9 4.4 软膏的性状鉴别10 4.5 软膏的稳定性检查10 4.6 小鼠日常活动状态和皮肤变化10 4.7 体外抑菌实验11 5 讨论13 参考文献15 综述17 致 谢23 家蝇幼虫蝇油的提取及其抗衰老作用研究 摘要：目的 对家蝇幼虫蝇油的提取进行优选，研究家蝇蝇油的抗衰老作用和体外抑菌效果 ，为化妆品的开发提供理论依据；方法 通过设计正交试验优选出家蝇幼虫蝇油的提取工艺；皮下注射D- 半乳糖和紫外灯照射小鼠背部皮肤造成皮肤衰老模型，同时设空白对照和模型 组研究蝇油的抗衰老作用；蝇油的体外抗菌效果；结果 70℃、2.5h，1:10料液比为最佳提取工艺；蝇油有一定的抗衰老作用；蝇油对大肠 杆菌、金色葡萄球菌和枯草杆菌均有一定的抑菌效果；结论 超声联合浸提法适合从家蝇幼虫中快速有效提取蝇油，蝇油有一定的抗衰老效 果和抗菌作用。 关键词：家蝇幼虫；蝇油；抗衰老；抗菌 The extraction of the grease from Musca domestica and the study of the effect in anti-aging Abstract: Objective To optimize the best way of the extraction of the grease from Musca domestica , its effect on anti-aging and explore the effect of the antibacterial ability, which provides the theoretical basis for the development in musmetic. Method Through design the orthognal test to optimize the way of the extraction of the grease from Musca domestica, produce the aged model by subcutaneous injection with D-galactose and shine the back in mice with UV light, and projecting blank control and modle set to study the grease from Musca domestica and the result of the antibacterial in the meantime. Result The optimal condition was petroleum ether as the extraction solvent, temperature 70℃, extraction time 2.5 hours, and solvent volume 12 ml/g. The grease can take effect in anti-aging and antibacterial ability among escherichia, staphylococcus aureus and bacillus subtilis. Conclusion The ultrasound combined extraction can be used in the extraction of the grease from effciently. And the grease is effective on anti-aging and antibacterial ability. Keywords: Musca domes