国内可购易转染细胞调查.docx

协和细胞中心肝细胞：IAR20小鼠肝细胞（国外建立）?non-transformed cell linerat liver epithelial cell line上皮细胞转染比较难one non- malignant lineJournal of Cell Science?122, 3883-3893 (2009)选择1 HEPA1-6小鼠肝癌细胞比较容易转染在这个细胞株上做糖尿病，文章发的也还可以Redox-dependent and ligand-independent?trans-activation of insulin receptor by globular adiponectin?，HepatologyVolume 46,?Issue 1,?pages 130–139,July 2007Long-Term Administration of Estradiol Decreases Expression of Hepatic Lipogenic Genes and Improves Insulin Sensitivity in ob/ob Mice: A Possible Mechanism Is through Direct Regulation of Signal Transducer and Activator of Transcription 3，Molecular Endocrinology 20 (6): 1287-1299CCC-HEL-1 人胚胎肝正常细胞(自建，排除)Hep G2 人肝癌细胞排除SMMC-7721人肝癌细胞国产的排除武汉细胞中心BALB/C小鼠肝癌细胞系（H22）鼠国内排除选择2GDC104大鼠肝癌细胞系H4-ⅡE鼠国外文章很多，做DM的比较多，也有不少文章是关于转染的国内做这个细胞想对比较少，论坛中讨论这个细胞转染的帖子都比较少人胎肝细胞系(L-02)人国内排除人肝癌细胞系(Bel-7402)人国内排除人肝癌细胞系(SSMC-7721)人国内排除人肝癌细胞系(Hep G2)人国外排除人肝癌细胞系(Hep-3B)人国外用的比较少的细胞草鱼肝脏细胞(L8824)鱼国内排除GDC132 鼠肝癌细胞（H22-H8D8）国内排除GDC134肝细胞系（Huh-7）国外选择3 Huh-7论坛上说这个细胞比较好转染，日本人建的细胞株，可以不依赖血清生长，肝癌细胞株，文章也发的不错Human hepatoma cell line established in Japan.Date accepted: 03/29/1985?Other name: HuH-7, JTC-39?Animal: human?Sex and Age: M :57 years old?Race: Japanese?Scientific Name: Homo sapiens?Case history: liver cancer?Classification: tumor?Tissue prepared: liver?Origin of tumor: liver?History of the cell: Okayama University = JCRB Cell Bank?Medium: Dulbeccos minimal essential medium with 10% fetal bovine serum. Refer Ref (1) for other medium with low serum concentration.?Passage method: Cells are treated with 0.2% trypsin and 0.02% EDTA.?CO2 Concentration: 5 %?Cell no. at passage: 2.5x10^4 cells/cm^2?Genetics: differenciatedhepatoma?Life span: infinite?Characteristics: hepatoma (differenciated), age 57, (M)?Cell ID data: available?DNA Profile: D5S818:12D13S317:10,11D7S820:11D16S539:10VWA:16,18TH01:7AmelogeninTPOX:8,11CSF1PO:10.3Established by Nakabayshi,H. Sato,J.?Deposited by Sato,J.?Special notice: No commercial purpose.?Cell bank: OUCI(JCR?References: (1,2,3,4)?