国外生物技术第5单元中文.doc

至今丁二酸主要用化学法生产。不过现在人们逐渐感兴趣利用可再生资源生产大宗化学品。在奶酪和牛奶生产中，乳清作为副产物大量产生。大多数乳清被处理了，但由于其高生物需氧量BOD而造成环境问题。玉米浆是作为副产物大量产生。大多数乳清被处理了，但由于其高生物需氧量BOD而造成环境问题。玉米浆是作为玉米淀粉生产中的副产品而获得。乳清含乳糖大约4.5%（w/v），蛋白质0.8%（w/v），盐1.0%（w/v）和乳酸0.1%-0.8%（w/v），因此是发酵工业生产很好的底物。好几种化学品，比如乳酸、乙醇、丙酸和聚3-羟基丁酯，都是乳清发酵生We recently separated from the rumen of cattle was able to use glucose yield succinic acid bacteria Mannheimia succiniciproducens MBEL55E.Preliminary research findings, Mannheimia succiniciproducens MBEL55E in adding yeast extract case also can be lactose as carbon source, since inference using whey as cheap raw materials for the preparation of succinic acid is feasible.The yeast extract is more expensive, we inspected to corn syrup as a complex nitrogen source possibilities.This paper reports the whey and corn pulp as raw material, the Mannheimia succiniciproducens MBEL55E by batch and continuous fermentation of succinic acid. 材料和方法Materials and methods 微生物及其生长条件Microorganisms and their growth conditions 用于丁二酸发酵的菌株为Mannheimia succiniciproducens MBEL55E（KCTC 0769BP）。细胞生长在含有100mL培养液的密封厌氧瓶中，培养基组成为（g/L）：3g K2HPO4,1g NACL,1g(NH4)2SO4,0.2g CACL2.2H2O,..........。培养液在充满氮气的厌氧瓶中121℃加热灭菌15分钟，然后以浓硫酸调节灭菌后的培养基中pH值为6.5，厌氧瓶中的n2用co2置换，同时加入。。。使其浓度达到1mg/L以确保其厌氧环境。15分钟后，在以上还原性培养液中接入1.5mL的在甘油中保存的菌种，于39℃培养12小时。For succinic acid fermentation strains of Mannheimia succiniciproducens MBEL55E (KCTC 0769BP).Cell growth in culture medium containing 100mL sealed anaerobic bottle, medium composition for (g / L): 3G K2HPO4, 1g NACL, 1g 2SO4, 0.2g CACL2.2H2O (NH4),...........Medium in nitrogen filled anaerobic bottle in 121 degrees Celsius heat sterilization for 15 minutes, then with concentrated sulfuric acid regulation of the sterilized culture medium pH value is 6.5, the anaerobic bottle N2 CO2 replacement, while adding...Make it reached 1mg / L to ensure its anaerobic environment.After 15 minutes, in the above reducing medium access 1.5mL in glycerine preserved species, at 39 degrees C were cultured for 12 hours. 对于摇瓶培养，对数生长期