基于HPLC-ECD法与聚类分析的中国白酒品质鉴别.docVIP

基于HPLC-ECD法与聚类分析的中国白酒品质鉴别 于静 孙翔宇 王宏镭 黄卫东 中国农业大学食品科学与营养工程学院 北京市产品质量监督检验院 X 关注成功！ 加关注后您将方便地在 我的关注中得到本文献的被引频次变化的通知！ 新浪微博 腾讯微博 人人网 开心网 豆瓣网 网易微博 摘????要： 白酒中的活性物质影响人的味觉, 是感官品评的重要指标之一, 库仑阵列高效液相色谱法能够检测白酒中电化学活性物质。首次建立了我国白酒的HPLC-ECD检测方法, 在最优色谱条件下, 8通道能采集固态法白酒中102个信息峰, 各物质得到了很好的分离, 方法精密度、稳定性高;样品无需前处理, 检测到的物质无需定性、定量分析, 方法简便快捷、成本低。使用该方法对固态法白酒与酒精勾兑白酒进行分析, 发现该方法能够检测到白酒中多种活性物质, 指纹图谱信息丰富, 两种白酒谱图差异明显。同时, 运用聚类分析对检测数据处理, 能实现准确区分固态法白酒与酒精勾兑白酒。运用该方法进一步分析不同勾兑比例的固液法白酒, 聚类结果与实际相符, 白酒样品被准确分为4类:固态法白酒含量比例分为10%、15%、75%和100%。表明该方法可以有效应用于白酒分类鉴定。 关键词： 白酒; 固态法白酒; 固液法白酒; 酒精勾兑白酒; 库仑阵列高效液相色谱法; 聚类分析法; Application of HPLC-ECD Combined with Cluster Analysis in the Identification of Chinese Spirits Quality YU Jing SUN Xiangyu WANG Honglei HUANG Weidong College of Food Science and Nutritional Engineering, China Agricultural University; Beijing Products Quality Supervision and Inspection Institute; Abstract： High performance liquid Chromatography-coulometric electrochemical array detection (HPLC-ECD) could be used to detected with electrochemical active substances. Meanwhile, these substances are the sensory substances of Chinese spirits, particularly in sensory evaluation. In this study, a method using HPLC-ECD on Chinese spirits was established for the first time. Under the optimal chromatographic conditions, when using 8 channel, 102 characteristic peak in Chinese spirits could be identified. All peaks are well separated with high stability and precision. The method was direct injection without pretreatment, and did not need to qualitative and quantitative analysis the detected substances, which means this method is simple fast, low cost and high efficiency. Using this method, the Chinese spirits by traditional fermentation and the Chinese spirits by alcohol blending could be distinguished. A variety of active substances in these two kinds Chinese spirits could be detected, the fingerprint information is rich, and there are obvious differences between the two kinds of Chinese spirits. What