2013年我院临床分离细菌分布及耐药性监测.docVIP

精品论文 参考文献 2013年我院临床分离细菌分布及耐药性监测 江秀全 戴海印 林燕青 蒋智锋 郭琳琳 王琳琳 （福建省龙岩市第二医院检验科院感科 364000） 【摘要】目的：了解我院2013年临床分离病原菌的分布特点及其对常用抗菌药物的敏感性和耐药性情况。方法：细菌分离按常规方法进行，抗菌药物敏感试验按CLSI2012年版进行并判断结果。结果：2637株非重复临床分离菌中：革兰阳性菌844株，占32.0%(844/2637)，革兰阴性菌1793 株，占68.0%（1793 /2637）。革兰阳性菌中，金黄色葡萄球菌为分离菌株的第一位。葡萄球菌属细菌中耐甲氧西林金葡菌（MRSA）和耐甲氧西林CNS（MRCNS）检出率分别为28.3 %和78.0%，万古霉素对革兰阳性球菌的抗菌活性最强，未发现万古霉素中介金葡菌（VISA）和耐万古霉素金葡菌VRSA菌株。屎肠球菌对测试药物的耐药率普遍高于粪肠球菌，未发现对万古霉素、替加??素、利奈唑胺的耐药株。革兰阴性菌中，大肠埃希菌位居第1位（占19.6%），肺炎克雷伯菌位居第2位。厄他培南对革兰阴性杆菌的抗菌活性最强，其次亚胺培南。鲍曼不动杆菌对亚胺培南和美罗培南耐药率分别为21.6%和66.7%。铜绿假单胞菌对头孢呋辛酯、头孢唑啉、头孢呋辛、呋喃妥因、头孢替坦、氨苄西林、头孢曲松、复方新诺明耐药率较高大于90%，并呈现多重耐药，对其他测试抗生素的耐药率均在20 %以下，对亚胺培南和美罗培南耐药率分别为5.9%和2.1%。结论：微生物检验为临床提供可靠用药依据，合理选用抗菌药物是治疗和预防细菌耐药的关键。 【关键词】耐药性 细菌 细菌耐药性监测 细菌药物敏感性试验 【中图分类号】R96 【文献标识码】A 【文章编号】2095-1752（2014）19-0392-03 Distribution and antibiotic resistance of bacterial pathogens isolated during the period 2013 in our hospital JIANGxiuquan DAIhaiyin LINyanqing ZHANGchunjun GUOlinlin JIANGzhifeng 【Abstract】 Objective：To investigate the distribution and antibiotic resistance of bacterial pathogens isolated during the period 2013 in our hospital.Methods： Clinical isolates were identified and subjected to antimicrobial susceptibility testing by means of a unified protocol. The results were analyzed according to CLSI breakpoints(2013).Results：Of the 2637 clinical isolates, gram positive and gram negative organisms accounted for32.0%(844 /2637 )and68.0 %(1793 /2637 ), respectively. Coagulase negative Staphylococcus (VISA)was the most frequently isolated gram positive bacteria. The prevalence of methicilin-resisitant strains was 28.3 % in Staphylococcus aureus(MRSA), and 78.0% incoagulase-negative Staphylococcus(MRCNS).Vancomycin was the most active antimicrobial agent against gram positive cocci. No BISA or VRSA strain was identified. E. faecium strains was more resistant to most of the antibiotics tested than E. faeca