TNF-α对损伤的内侧副韧带和前交叉韧带中.docVIP

精品论文 参考文献 TNF-α对损伤的内侧副韧带和前交叉韧带中 李杰 　　（四川省成都市龙泉驿区第一人民医院骨科四川成都610100） 　　【摘要】目的：就TNF-alpha;对损伤的内侧副韧带和前交叉韧带中BMP-1基因表达的影响进行探讨。方法：选择重庆医科大学附属第一医院骨科教研室在2011年09~11月时间内所收集到的4例滑膜组织和交叉韧带标本，其中女2例，男2例，截肢膝关节相应组织是在无菌条件下用镊子和剪刀来取得。先细胞培养，其次，pET32a(+)-Tat-PTD-VP3重组质粒构建示，分别测定半定量PCR、实时定量PCR。结果：本组资料研究表明，TNF-alpha;作用时间分别为24h、12h、6h、2h，作用浓度为5ng/ml。对于那些已经损伤的MCL细胞中，BMP-1的表达随着TNF-alpha;浓度的不断增加也会出现逐步的下降趋势，用实时定量PCR来进行显示，BMP-1表达为117%、70%、62%、41%。而对于那些已经损伤的ACL细胞中，BMP-1的表达随着TNF-alpha;浓度的不断增加会出现逐渐下降的趋势。用实时定量PCR来进行显示，BMP-1表达从最初的69%，下降到最后的27%。结论：TNF-alpha;能够有效地下调ACL作用，以及前交叉韧带（ACL）和内侧副韧带（MCL）中BMP-1基因表达都出现了较大的下降，本实验为有效促进ACL自我修复，早期抑制TNF-alpha;浓度提供了较好的理论依据。 　　【关键词】TNF-alpha;；内侧副韧带；前交叉韧带；BMP-1；基因表达Effects of BMP-1 gene expression of TNF- on injury of the medial collateral ligament and anterior cruciate ligament. 　　Li Jie(Department of orthopedics, the first peoples Hospital of Longquanyi District, Chengdu City, Sichuan Province, Sichuan, Cheng Du, 610100) 　　【Abstract】Objective:To explore effects of BMP-1 TNF- on the injury of the medial collateral ligament and anterior cruciate ligament in gene expression.Methods:4 cases of synovial tissue Department of orthopedics,First Affiliated Hospital of Chongqing Medical University collected in 2011：09~11 cruciate ligament specimens,including 2 cases of female,male 2 cases,amputation of knee joint corresponding tissue is achieved with tweezers and scissors under sterile conditions.Second,cell culture,pET32a (+) to construct the recombinant plasmid of -Tat-PTD-VP3,semi-quantitative PCR,real-time quantitative PCR were measured.Results:the study shows that,TNF- alpha; action time were 24h,12h,6h,2h,concentration is 5ng\/ml.For those who have damage in MCL cells,the expression of BMP-1 with increasing TNF- concentration will gradually decline,real-time quantitative PCR was used to display,BMP-1 expression was 117%,70%,62%,41%.And to all those who have injured ACL cells,the expression of BMP-1 with increasing TNF- concentration will gradually decline.Real-time quantitat