克感利咽口服液挥发油提取工艺与质量研究.docVIP

精品论文 参考文献 克感利咽口服液挥发油提取工艺与质量研究 劳燕霞 　　（广州王老吉药业股份有限公司 广东广州 510450） 　　【摘要】目的 优化克感利咽口服液挥发油提取工艺，并对挥发油进行薄层鉴别和高效液相含量测定。方法 采用水蒸气蒸馏法提取挥发油，以挥发油收得率和挥发油中胡薄荷酮含量的综合得分为考察指标，选择提取前浸泡温度、浸泡时间及提取时间为考察因素，运用正交试验优选工艺条件；采用薄层色谱法对挥发油中薄荷脑进行定性鉴别，高效液相色谱法对挥发油中胡薄荷酮进行定量测定。结果 优化的工艺条件是浸泡温度60℃、浸泡时间60分钟、提取2.5小时；薄层色谱鉴定薄荷脑特征性强；色谱条件：色谱柱：Phenomenex Luna（250mmtimes;4.6mmtimes;5mu;m），流动相：甲醇：水(70∶30)，流速：1ml/min，柱温：35℃，检测波长为252nm，胡薄荷酮在2.9212～186.955mu;g之间呈良好的线性关系，回收率为 99.10% 。结论 提取工艺合理、可行；建立的薄层鉴别方法及HPLC定量方法准确、简便。 　　【关键词】克感利咽口服液 挥发油 提取工艺 薄荷脑 胡薄荷酮 薄层鉴别 高效液相色谱法 　　【中图分类号】R94 【文献标识码】A 【文章编号】2095-1752（2014）10-0048-02 　　 　　Studies on the extraction process and quality of volatile oil of Kegan Liyan Oral Liquid 　　LAO Yan-xia 　　(Guangzhou Wang Lao Ji Pharmaceutical Company Limited,Guangzhou 510450,China) 　　【Abstract】 Objective To optimize the extraction technology of volatile oil of Kegan Liyan Oral Liquid,and then qualitative and quantitative analysis of volatile oil were measured by thin layer chromatography ( TLC) and high performance thin layer chromatography ( HPLC) respectively. Methods steam distillation were used in the experiment,which was performed with volatile oil yield and pulegone content as the index and the soaking temperature,the soaking time,the time of immersion as affecting factors,then optimizing process condition by orthogonal test.The qualitative identification of menthol was performed by TLC,and the quantitative determination of Pulegone by HPLC.Results Optimal conditions were soak temperature of 60 ℃, soak time of 60 minutes, extraction time of 2.5 hours; identification of menthol by TLC was characteristic strong;the separation was performed on Phenomenex Luna（250mmtimes;4.6mmtimes;5mu;m）.The mobile phase consisted of methanol-water (70:30) at the flow rate of 1mL/min.The column temperature was 35℃ and wavelength was set at 252nm. there was a good linear relationship in the scope of 2.9212～186.955mu;g for pulegone , The recov