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Effects of light quality on rooting of Oncidium and Phalaenopsis plantlets25完
Effects of light quality on rooting of Oncidium and Phalaenopsis plantlets
Abstract：Effects of light quality of light-emitting diode (LED) on rooting of Oncidium and Phalaenopsis plantlets in vitro were studied. Fluorescent light was used as the control. The results showed that, red LED was in favor of enhancing plant height and synthesizing soluble sugar, starch and carbohydrate; the content of chlorophyllb, chlorophyll a + b and carotenoid of shoots including content of amino acid of plantlets in vitro were significantly increased by blue LED; complex of red, blue and yellow LED, Oncidium and Phalaenopsis plantlets all had strong growth, high enriched degree, more quantity roots and higher dry and fresh mass. The results indicated that, as compared with fluorescent light, application of complex of red + blue + yellow LED was propitious to improve propagation coefficient, breed vigorous plantlets and decrease energy consumption.
Key words：light-emitting diode; light quality; Oncidium; Phalaenopsis; rooting
1.introduction
Orchid is an important cultivated plant in horticulture flower and it has been cultivated for nearly two thousand years in China.Orchidaceae are quite valuable in ornamentation , but also in medicine , research and ecology.Becuse of its low reproductive rate and long period , micropropagation has become one of the most efficient means in orchid multiplication.
Light provides radiant energy for photosynthesis, furthermore, it provides signals used to regulate plant development process. Light quality and quantity have a profound in?uence on the growth and development of propagules cultured in vtro.Among them , the light quality on morphogenesis, growth and the regulation of metabolism of plantlets in recent years has become a hot research field of tissue culture.
Fluoresent tubes/lamps have been generally used for in vitro culture. However, these sources contain unnecessary wavelengths that are of low quality for promoting growth. Compared with those
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