生物化学(中国农业大学-刘国琴)第19章 基因工程简介.pdfVIP

第十九章基因工程简介 • 位点专一性重组 一. 目的基因 二. 基因载体 噬菌体 质粒 粘粒 三. 受体细胞 四. 基因工程的应用 限制性内切酶 逆转录PCR A0092201.mov 鸡输卵管上皮细胞特异性表达载体的构建 鸡输卵管上皮细胞特异性表达载体的构建 The list of eukaryotic expression vectors constructed The list of eukaryotic expression vectors constructed No. Name Promotor Target gene No. Name Promotor Target gene 1 pEGFP-N1 CMV IE EGFP 1 pEGFP-N1 CMV IE EGFP pEGFP pEGFP 2 CMV IE Lysozyme-EGFP 2 CMV IE Lysozyme-EGFP Lysozyme Lysozyme 3 pOVAG Oval-promotor EGFP 3 pOVAG Oval-promotor EGFP Oval-signal Oval-signal 4 pOVAG-2 Oval-promotor 4 pOVAG-2 Oval-promotor peptide；EGFP peptide；EGFP 5 pOVALG Oval-promotor Lysozyme-EGFP 5 pOVALG Oval-promotor Lysozyme-EGFP Oval-promotor Oval-promotor 6 pEOVALG Lysozyme-EGFP 6 pEOVALG Lysozyme-EGFP SV40 enhancer SV40 enhancer Expression of GFP gene in different vectors Expression of GFP gene in different vectors in Chicken Embryonic Fibroblasts (CEFs) in Chicken Embryonic Fibroblasts (CEFs) .pEGFP-Lyso