Bcl-22蛋白的表达而抑制细胞凋亡.PDFVIP

·1456 · doi 10.3969/j.issn. 1673-4254.2017.11.05 J South Med Univ, 2017, 37(11): 1456-1460 基础研究 辛伐他汀通过上调动脉粥样硬化大鼠血管壁辛伐他汀通过上调动脉粥样硬化大鼠血管壁Bcl-Bcl-22 蛋白的表达蛋白的表达 而抑制细胞凋而抑制细胞凋亡亡 胡司淦，李 辉，康品方，陈天平，李妙男，朱 建，高大胜，张 恒，王洪巨 蚌埠医学院第一附属医院心血管科，安徽 蚌埠 233004 摘要：目的探讨辛伐他汀对动脉粥样硬化（AS）大鼠模型血管壁细胞凋亡、Bcl-2 蛋白的表达干预作用。方法 高脂饲料及腹腔 注射维生素D3 复制大鼠AS 模型，随机分为对照组（n= 10）给予普通饲料喂养，实验组（n= 13）给予高脂饲料喂养，干预组（n= 13） 给予高脂饲料喂养基础上加用辛伐他汀干预，11周后取胸主动脉，观察其斑块变化。采用免疫组化Elivision 法测定AS 血管壁 中Bcl-2 蛋白表达。通过末端脱氧核苷酸转移酶介导的dUTP 缺口末端标记（TUNEL）法检测细胞凋亡指数（AI），分析各组中 Bcl-2 表达及AI 变化。结果 与对照组比较，Bcl-2 蛋白的表达在实验组明显降低（P0.05）；而与实验组比较，干预组Bcl-2 蛋白 的表达明显升高（P0.05），且与对照组比较无差异。同时，AI 在各组中的变化比较则相反，差异均有统计学意义（P0.05）。结 论 辛伐他汀抗大鼠AS 的作用可能与上调Bcl-2 蛋白表达，进而抑制细胞凋亡相关。 关键词：动脉粥样硬化；细胞凋亡；辛伐他汀；Bcl-2 ；免疫组织化学 Effects of simvastatin on aortic vascular endothelial cell apoptosis and Bcl-2 protein expression in a rat model of atherosclerosis HU Sigan, LI Hui, KANG Pinfang, CHEN Tianping, LI Miaonan, ZHU Jian, GAO Dasheng, ZHANG Heng, WANG Hongju Department of Cardiovascular Medicine, First Affiliated Hospital, Bengbu Medical College, Bengbu 233004, China Abstract: Objective To explore the effects of simvastatin on vascular endothelial cell apoptosis and Bcl-2 protein expression in the aorta in a rat model of atherosclerosis. Methods Thirty-six rats were randomized into control group (n=10), atherosclerosis model group (n=13) and simvastatin intervention group (n=13). In the latter two groups, rat models of atherosclerosis were established by intraperitoneal injection of vitamin D3 combined with high-fat feeding for 6 weeks, and the control rats were fed with regular diet. In the intervention group, the rats were further fed with high-fat diet with daily simvastatin treatment for 4 weeks. After the treatments, the pathological changes and plaque in the thoracic aorta were observed, and the expression of Bcl-2 protein was d