AOCS Cd 1-25 油脂的碘价测定.pdfVIP

SAMPLING AND ANALYSIS OF COMMERCIAL FATS AND OILS AOCS Official Method Cd 1-25 Withdrawn (Historical Interest Only) Iodine Value of Fats and Oils Wijs Method DEFINITION The iodine value is a measure of the unsaturation of fats and oils and is expressed in terms of the number of centigrams of iodine absorbed per gram of sample (% iodine absorbed). SCOPE Applicable to all normal fats and oils that do not contain conjugated double bonds (see Notes, 1). Applicable to all normal fats and oils with samples having iodine values between 0–15. APPARATUS 1. Glass-stoppered iodine flasks—500 mL. 2. Glass-stoppered volumetric flasks—1000 mL, for preparing standard solutions. 3. Pipet—25 mL, for accurately dispensing 25.0 mL of Wijs solution. 4. Volumetric dispenser—20 mL, 1 mL adjustability, for 10% potassium iodide (KI) solution. 5. Volumetric dispenser—2 mL, 1 mL adjustability, for starch solution. 6. Volumetric dispenser—50 mL, 1 mL adjustability, for distilled water. 7. Repeater pipet—with filling flask, 20 mL, for cyclohexane. 8. Analytical balance—accurate to ±0.0001 g. 9. Magnetic stirrer. 10. Filter paper—Whatman no. 41H, or equivalent. 11. Beakers—50 mL. 12. Hot air oven. 13. Timer. REAGENTS 1. Wijs solution—see Notes, 2 and Caution. 2. Potassium iodide (KI)—reagent grade. 3. Carbon tetrachloride—reagent grade (see Notes, Caution and Recommendations). The absence of oxidizable matter in this reagent is verified by shaking 10 mL of the reagent with 1 mL of saturated aqueous potassium dichromate solution and 2 mL of concentrated sulfuric acid; no green coloration should appear. 4. Soluble starch solution—recently prepared, tested for sensitivity (see Notes, 3). Make a paste with 1