miR-21,Sprouty1功能轴调控绝经后骨质疏松患者骨髓间充质干细胞的成骨能力.doc

miR-21/Sprouty1功能轴调控绝经后骨质疏松患者 骨髓间充质干细胞的成骨能力 杨 楠1，周 威1，王 光2，丁 寅3，金 岩4(1解放军第309医院口腔科，北京市 100091；2解放军91551部队门诊部，江西省九江市 332005；解放军第四军医大学口腔医院，3正畸科，4组织工程中心，陕西省西安市 710032) 引用本文：杨楠，周威，王光，丁寅，金岩. miR-21/Sprouty1功能轴调控绝经后骨质疏松患者骨髓间充质干细胞的成骨能力[J].中国组织工程研究，2017，21():3287-3292. DOI:10.3969/j.issn.2095-4344.2017.21.002 ORCID: 0000-0003-0605-2250(杨楠) 文章快速阅读： 文题释义： miRNA：是一类具有18-25个核苷酸的非编码RNA，它通过与靶基因3’UTR区非完全配对来抑制靶基因的翻译和稳定，参与多种生物功能。 Runx2和Osterix与骨髓间充质干细胞的成骨分化miR-21/Sprouty1 function axis regulates the osteogenic differentiation of bone marrow mesenchymal stem cells after postmenopausal osteoporosis Yang Nan1, Zhou Wei1, Wang Guang2, Ding Yin3, Jin Yan4 (1Department of Stomatology, the 309th Hospital of PLA, Beijing 100091, China; 2Outpatient Department of Troop 91551, Jiujiang 332005, Jiangxi Province, China; 3Department of Orthodontics, Stomatology Hospital of Fourth Military Medical University of PLA, Xi’an 710032, Shaanxi Province, China; 4Center of Tissue Engineering, Fourth Military Medical University of PLA, Xi’an 710032, Shaanxi Province, China) Abstract BACKGROUND: Osteogenic differentiation is a complex process involving transcriptional and post-transcriptional regulation by multiple signaling pathways, and the specific mechanisms remain unclear. It is of great significance to study the role of critical miRNAs in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in the treatment of osteoporosis and bone defects. OBJECTIVE: To explore the regulatory ability of miR-21/Sprouty1 function axis in the osteogenic differentiation of BMSCs in patients with postmenopausal osteoporosis. METHODS: BMSCs were isolated from healthy people (H-hBMSCs) and patients with postmenopausal osteoporosis (PMOP-hBMSCs), and their osteogenic ability was compared. Expression of miR-21 and Spry1 at gene and protein levels was detected by real-time RT-PCR and western blot assay, respectively. miR-21 expression was upregulated via transfection in PMOP-hBMSCs,