HCV和HIV-1合并感染标本中HCV基因分型.docVIP

HCV和HIV-1合并感染标本中HCV基因分型 HCV和HIV-1合并感染标本中HCV基因分型 2010-01-11 钟平　段继良　刘桂珍　朱为　张伊璜 　　【摘要】　目的　了解丙型肝炎病毒(HCV)和人类免疫缺陷病毒I型(HIV-1)混合感染标本中HCV基因型情况。方法　采用了根据HCV 5′端非转译区基因组所设计的反相探针杂交测定方法(INNOLiPA,HCVⅡ)，对来自六个地区的17份抗-HCV和抗-HIV-1双阳性标本进行HCV基因型分型研究。结果　在17份标本中，7份(41.18%)为I(1b)型病毒，5份(29.41%)为Ⅱ(2a/2c)型病毒，5份(29.41%)为I型(1b)和Ⅱ(2a/2c)混合型病毒。结论　发现HCV与HIV-1合并感染标本中存在I(1b)型、Ⅱ(2a/2c)型以及I(1b)型与Ⅱ(2a/2c)型混合型HCV。值得注意的是对α-干扰素治疗不敏感并且较易趋于导致严重的慢性肝炎、肝硬化和肝细胞癌的Ⅱ型HCV感染的比率占70.58%(12/17)。 　　【关键词】　肝炎病毒，丙型　　人类免疫缺陷病毒感染　　基因型 Genotyping of HCV in the HCV and HIV-1 co-infected samples　Zhong Ping,Duan Jiliang,Liu Guizheng,et al.　Shanghai Institute of Biological Products,Shanghai 200052 　　【Abstract】　Objective　To investigate the genotypes of HCV in the HCV and HIV-1 co-infected samples.Methods　Genotypes of HCV were determined by the INNO-LiPA HCVII kit based on a reverse-hybridization technique which was used for genotyping HCV and determining the variation in the 5′ untranslated regions of different HCVs.Seventeen of HCV and HIV-1 co-infected samples derived from 6 different high-risk geographic areas in China were studied.Results　The results indicated that 7/17(41.18%) of samples were found to be of (1b) genotype,5/17(29.41%) were Ⅲ(2a/2c) genotype and 5/17(29.41%) were mixed-genotype with Ⅱ (1b) and Ⅲ (2a/2c).Conclusion　The presence of HCV Ⅱ(1b) and Ⅲ(2a/2c) genotype as well as HCV Ⅱ and Ⅲ mixed genotype were found in HCV and HIV-1 co-infected samples.Notably,HCV Ⅱ (1b) genotype,usually fails to respond to IFN-α is apt to proceed much faster to severe chronic hepatitis,cirrhosis and hepatocellular carcinoma,had a significantly higher prevalence of 12/17 (70.58%) than other subtypes in this study. 　　【Key words】　HCV HIV　　Infection　　Genotype 　　丙型肝炎病毒(HCV)是引起绝大多数非甲非乙肝炎的致病原［1］。HCV至少存在6个主要的基因型以及它们的基因亚型［2］，另外，HCV基因型分布有明显的地区性［3］。有证据表明感染Ⅱ型HCV的患者，对α-干扰素治疗不敏感，并较易发展成为严重的慢性肝炎、甚至导致肝硬化和肝细胞癌症［4～6］。HCV与HIV-1合并感染血清/血浆中的HCV基因型分型的研究在国外已有报道［7，8］。本研究旨在了解从国内不同地区高危人群中HCV和HIV-1合并感染标本中的HCV基因型的情况，这无论对HCV和HIV-1合并感染的自然史和分子流行病调查，还是对HCV和HIV-1