猪传染性肠胃炎病毒TGEV在ST 细胞中的增殖规律研究.pdfVIP

J069 猪传染性肠胃炎病毒(TGEV)在 ST 细胞中的增殖规律 1 1 1 2 1 周燕 王建超 华平 邹勇 谭文松 1 ( 华东理工大学生物反应器工程国家重点实验室，上海200237； 2 上海市农业科学院兽牧兽医研究所，上海201106) 摘 要：为了建立病毒疫苗高效工业化生产过程，通过微载体和转瓶培养系统，研究了 MOI、TOI 和病毒维持液组成对细胞生长和病毒繁殖的影响。MOI 的大小决定着病毒对细胞的感染方式，影 响细胞失活速率和单位细胞病毒产量；TOI 决定病毒感染时细胞密度和细胞生理状态，影响细胞 对病毒的敏感程度以及胞内病毒的增殖效率；丰富的维生素、氨基酸等物质有利于病毒滴度的提 高。采用微载体和生物反应器系统生产 TGEV，病毒滴度可达 11 lgTCID50·0.2ml-1 ，比方瓶培养系 统提高约100 倍。 关键词：ST细胞 猪传染性肠胃炎病毒（TGEV） 微载体 生物反应器 OPTIMIZATION OF TRANSMISSIBLE GASTROENTERITIS VIRUS (TGEV) PRODUCTION IN ST CELL 1 1 1 2 1 ZHOU Yan ， WANG Jianchao ， HUA Ping ， ZHOU Yong and TAN Wensong 1 ( State Key Lab of Bioreactor Engineering, ECUST, Shanghai 200237, China; 2 Institute of Veterinary Research, Shanghai Academy of Agriculture, Shanghai 201106, China) Abstract Multiply of infection (MOI), Time of infection (TOI) and medium composition that affect viral infection and replication efficiency were investigated for the development of high efficient processes of viral vaccine production on microcarrier in spinner bottle. Based on the understanding of parameters ruling cell-virus interactions, it was found that MOI played a determinant role on the type of virus infection (synchronous infection or secondary infection), the cell death rate and the virus productivity per cell. TOI affected the number of viable cells at the point of infection, sensitivity of cells to virus and virus productivity per cell. The titer of viral vaccine produced by the cells cultured on microcarriers in a biorea