双歧杆菌对内毒素损伤幼鼠肠道防御素-5表达的影响-f6publishing.docVIP

Name of journal: World Journal of Gastroenterology ESPS Manuscript NO: 14137 Columns: ORIGINAL ARTICLE Basic Study Effect of bifidobacterium on defensin-5 expression in intestinal injury of preweaning rats Wang W et al. Pretreatment with bifidobacterium Wei Wang, Shu-Fen Yang, Li-Hong Ren, Xiu-Xiu Zhang, Shui-Lian Yu Wei Wang, Shu-Fen Yang, Li-Hong Ren, Xiu-Xiu Zhang, Shui-Lian Yu, Department of Pediatrics, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China Author contributions: Wang W designed and performed research, analyzed data and wrote the paper; Yang SF designed and performed research; Ren LH designed research and analyzed data; Zhang XX contributed new reagents or analytic tools; Yu SL contributed new reagents or analytic tools. Supported by the Foundation of Education Department of Heilongjiang Province, No.Correspondence to: Wei Wang, MD, Department of Pediatrics, the Second Affiliated Hospital of Harbin Medical University, No. 246 Xuefu Road, Nangang District, Harbin 150086, Heilongjiang Province, China. wendywang_1972@126.com Telephone: +86-451 Fax: +86-451Received: September 21, 2014 Revised: October 29, 2014 Accepted: December 5, 2014 Published online: Abstract Aim: To investigate the protective effect of bifidobacterium in endotoxin-induced intestinal injury in preweaning rats. METHODS: Preweaning rats were randomly divided into three groups (n = 40 for each): a control group (group C), a model group (group E) and a treatment group (group T). Both groups E and T were intraperitoneally injected with lipopolysaccharide (LPS) which dose was 5 mg/kg (5 mg/L in normal saline), and group T was intragastrically administrated with bifidobacterium suspension (2.0 × 109 CFU/mL, 0.5 mL each time, twice a day, until the end of the experiment) 7 d before LPS administration. Group C was intraperitoneally injected with normal saline. After intraperitoneal injection and int