液相色谱柱养.ppt

流动相平衡至基线稳定，进样测试。 ① 水 四氢 呋喃 （THF） 二氯甲烷 ② ③ ④ ⑤ ⑥ Chiral HPLC Column若为强保留物质污染，清洗与再生程序示意图： 3.5亲水性色谱柱（Hydrophilic chromatographic column，HILIC） 一般同正相色谱柱，可参考氨基柱与氰基柱的处理方法； 亦可根据色谱柱的可能污染情况，合理选择溶剂冲洗与再生。 3.6氨基酸分析柱（Venusil AA） 一般同C18柱，可参考C18柱的处理方法； 亦可根据色谱柱的可能污染情况，合理选择溶剂冲洗与再生。 * Potential zero point and sensitivity drifts caused by the erratic warm-up process are eliminated However, warm is not needed with balance having a stand-by current loop provided they are not separated from power. * Before you open the weighing chamber, take a look at the display: it must be exactly zero. WHY? Any existing zero point error will become part of the measuring. Never touch either the tare container or the sample with bare hands - use a forceps or another adequate tool. WHY? Body contact may change the temperature and the humidity of the sample and the container. * Never put your hand inside the weighing chamber - make sure your forceps is long enough so your hand stays outside WHY? Your hand gives off moisture and heat. * Never put your hand inside the weighing chamber - make sure your forceps is long enough so your hand stays outside WHY? Your hand gives off moisture and heat. * Open the windows of the weighing chamber only long enough and wide enough to permit easy loading of the container and the sample WHY? Every time you open the weighing chamber, you may cause temperature changes and turbulence inside. * Set your sample in the center of the pans. WHY? Off-center loading may cause corner load errors. * Close the weighing chamber promptly after sample or container loading The longer the weighing chamber is kept open, the greater the chance that the temperature and humidity inside changes and turbulence are caused Take your reading as soon as the result is stable Your sample may give off or absorb moisture and thus lose or gain weight. Remove the sample from the weighing chamber as soon as you have read the result and close the window If your sample remains on the pan for length of tim