一种新型致小鼠拟衰老模型的初步建立-海南医学.pdfVIP

Vol.23 No.5 March 2012 doi:10.3969/j.issn.1003-6350.2012.05.007 ·实验研究· 一种新型致小鼠拟衰老模型的初步建立 1 2 龙金烈 ，杨 明 (1. 自贡市第四人民医院检验科，四川 643000 ； 2.四川大学生命科学学院，四川 成都 610041) 【摘要】 目的 研究长期高剂量葡萄糖对小鼠衰老情况的影响。方法 将健康小鼠按体重分段法随机分 为：对照组(注射生理盐水)、D-半乳糖组、D-半乳糖+葡萄糖组、葡萄糖组，每日颈背部皮下注射相应药物建立衰老模 型，连续14周观察及检测相关衰老指标。结果 D-半乳糖组、D-半乳糖+葡萄糖组、葡萄糖组小鼠行为较迟缓、反应 较迟钝、较嗜睡(P0.05) ；葡萄糖组小鼠红细胞脆性最大、物体识别分辨能力最低、皮肤羟脯氨酸含量最低、脑组织 MDA 含量最高(P ＜0.05)。结论 高剂量葡萄糖长期反复注射对昆明小鼠有致拟衰老的作用，且此作用似乎优于传 统的D-半乳糖模型。本实验为建立一种新型致小鼠衰老模型提供了初步的实验基础。 【关键词】 葡萄糖；D-半乳糖；衰老模型 【中图分类号】R-332 【文献标识码】A 【文章编号】 1003—6350（2012 ）05—020—05 Contruction of glucose-induced aging model in KM mice. LONG Jin-lie 1, YANG Ming ２. 1. Department of Clinical Laboratory, The Forth People ’s Hospital of Zigong City, Zigong 643000, Sichuan, CHINA; 2. School of Life Science, Sichuan University, Chengdu 610041, Sichuan, CHINA 【Abstract 】 Objective To study the effect of high dose of glucose on the aging of mice for a long time. Methods The KM mice were randomly divided into four groups by weight: the control group (n=8, injected with sa- line), D-galactose group (n=7, injected with D-galactose), D-galactose+glucose group (n=7, injected with D-galactose and glucose), and glucose group (n=7, injected with glucose). The mice in the four groups were given corresponding drugs everyday by cervicodorsal hypodermic injection for fourteen weeks. The indicators related to aging were ob- served and measured. Results Mice in D-galactose group, D-galactose + glucose