整合子在鲍曼不动杆菌中的分布与结构分析.docVIP

精品论文 参考文献 整合子在鲍曼不动杆菌中的分布与结构分析 杨丽萍 伊茂礼 阎志勇（山东省烟台毓璜顶医院 山东烟台 264000） 【中图分类号】R378【文献标识码】A【文章编号】1672-5085（2011）44-0108-03 【摘要】目的 了解我院临床分离的鲍曼不动杆菌的整合子携带情况，以及整合子与鲍曼不动杆菌的耐药相关性分析。方法 用VITEK2 Compact全自动微生物分析系统及纸片扩散法检测临床分离的鲍曼不动杆菌对常用22种抗生素的敏感性。PCR扩增整合酶基因检测鲍曼不动杆菌携带整合子情况，同时对整合子可变区进行扩增及测序以其了解整合子耐药基因盒携带耐药基因的情况。结果 鲍曼不动杆菌呈多重耐药性。鲍曼不动杆菌对CPZ/SB耐药率为1.2%，对替加环素（15.4%）、左旋氧氟沙星（34.4%）、亚胺培南（54.3%）、比阿培南（49.4%）、阿米卡星（59%），对其他抗生素均在71%以上。104株中有73株菌株含Ⅰ类整合子（阳性率为70.2%），未有Ⅱ、Ⅲ类整合子扩增阳性菌株，且Ⅰ类整合子阳性株的耐药性均高于阴性株。Ⅰ类整合子基因盒序列分析表明我院鲍曼不动杆菌Ⅰ类整合子携带aacA4、catB8和aadA1 3种耐药基因。结论 我院分离的鲍曼不动杆菌主要携带Ⅰ类整合子，且与多重耐药性密切相关。 【关键词】鲍曼不动杆菌 整合子 整合酶 多重耐药性 【Abstract】 Objective To investigate the drug resistance spectrum of baumanii strains isolated from clinical samples from our hospital and study the correlation between the existence of classⅠintegron and drug resistance of baumanii. Methods Minimal inhibitory concentration (MIC) experiment was taken to examine the resistance of baumanii to 22 kinds of antibiotics regularly used in our hospital. Polymerase chain reaction(PCR) was proceeded by amplifing the variable domain of classⅠintegron to examine the existence of classⅠintegron and to find out which drug-resistant gene was contained in it.Results Baumanii strains isolated from our hospital had a wide drug-resistance spectrum: CPZ/SB (1.2%)， tigecycline (15.4%) levofloxacin (34.4%) imipenem (54.3%) Biapenem (49.4%) amikacin (59%)， all of the other 16 kinds of antibiotics (gt;71%).70.2% (73/104) of baumanii strains contained classⅠintegron. Drug resistance specturum of strains with classⅠintegron was wider than strains without it. 3 kinds of drug-resistant gene : aacA4， catB8 and aadA1 located in classⅠintegron which carried by some of baumanii strains isolated from our hospital. Conclusion There was a tight correlation between the existence of classⅠintegron and multidrug resistance of baumanii. 【Key words】Acinetobacter baumannii Multidrug resistence Integron Integrase 鲍曼不动杆菌导致的多种感染流行或爆发的医院感染在过去10年里已在世界各地包括