猪圆环抗体PC-Ab试剂盒使用方法.DOCVIP

猪圆环抗体（PC-Ab）试剂盒使用实验原理本试剂盒应用双夹心法测定中。用纯化的包被微孔板，制成固相抗，，经过彻底洗涤后底物TMB显色。TMB在酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。用酶标仪在nm波长下测定吸光度（OD值）试剂盒组成 酶板稀释液-20℃保存，但应避免反复冻融操作步骤 μl。然后在待测样品孔μl，然后再加待测样品10μl。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻混匀，分钟甩干μl，空白孔除外。 μl，再加入显色剂B50μl，轻轻震荡混匀，37℃避光显色15分钟. 终止：每孔加终止液50μl，终止反应。450nm波长依序测量各孔的度（OD值）≥1.00; 阴性对照平均值≤0.10 临界值（CUT OFF）计算：临界值=阴性对照孔平均值+0.15 阴性判定：样品OD值 临界值（CUT OFF）者为猪圆环抗体（PC-Ab）阴性 阳性判定：样品OD值≥ 临界值（CUT OFF）者为猪圆环抗体（PC-Ab）阳性 。 浓洗涤液会有析出，稀释时可在水浴中加温助溶1．；2-8。 HP FOR RESEARCH USE ONLY 96 determinations Purpose This kit allows for the determination of HP concentrations in Human serum, and other biological fluids. Principle of the assay The kit assay HP level in the sample，use Purified antibody to coat microtiter plate wells, make solid-phase antibody, then add HP to wells, Combined With HP, after washing and removing non-combinative antibody and other components ,then Combined HP antibody which with HRP labeled become antibody – antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge HP exist in the sample or not. Materials provided with the kit 1 wash solution 20ml×1bottle 7 Stopp Solution 6ml×1 bottle 2 HRP-Conjugate reagent 6ml×1 bottle 8 Positive control 0.5ml×1 bottle 3 Microelisa stripplate 12well×8strips 9 Negative control 0.5ml×1bottle 4 Sample diluent 6ml×1 bottle 10 Instruction 1 5 Chromogen Solution A 6ml×1 bottle 11 Closure plate membrane 2 6 Chromogen Solution B 6ml×1 bottle 12 Sealed bags 1 Specimen requirements extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can b