溃疡性结肠炎患者结肠黏膜抗氧化反应元件结合活性及血红素氧合酶-1表达的变化.docVIP

精品论文 参考文献 溃疡性结肠炎患者结肠黏膜抗氧化反应元件结合活性及血红素氧合酶-1表达的变化 DOI：10.7504/nk2016010203 中图分类号：R587.1 文献标识码：A 　　摘要：目的: 检测UC患者结肠黏膜中ARE的结合活性及HO-1表达的变化, 分析ARE、HO-1的表达与UC病情严重程度的相关性. 　　方法: 选取UC活动期患者为病例组, 同时期结肠息肉患者为正常对照组. 结肠镜下取直肠-乙状结肠交界处黏膜组织4块. 分别用EMSA法测定ARE结合活性, 免疫组织化学染色方法测定HO-1蛋白表达. 　　结果: UC各组ARE结合活性均高于对照组, 依次为：重度UC组gt;轻中度UC组gt;对照组. UC组结肠黏膜组织中HO-1蛋白表达水平明显高于正常对照组, 依次为：重度UC组gt;轻中度UC组gt;对照组。相关性分析：UC患者中ARE结合活性的变化与HO-1表达的变化呈正相关。 　　结论: 随着溃疡性结肠炎患者病情的进一步加重, 结肠黏膜组织中ARE结合活性不断增强, HO-1表达也不断增加, ARE与HO-1的关系呈正相关, ARE的结合活性及HO-1的表达强度在一定程度上反映UC的病情严重程度. 　　关键词: 溃疡性结肠炎, 抗氧化反应元件, 血红素氧合酶-1, 凝胶电泳迁移率分析实验 　　Antioxidant Response Element Binding Activity and heme oxygenase-1 Expression in Colonic Mucosa of Ulcerative Colitis Patients 　　Rui Zhang,Li Liu, Hui Tian ,Dong-Qiang Zhao 　　AIM:To detect the ARE binding activity and HO-1 expression in colonic mucosa of UC patient and analyze the respectively revelance of ARE, HO-1 with severity of UC. 　　METHODS: This study collected 42 patients who are diagnosed as active ulcerative colitis as case group and 25 patients with colonic polyps as control group. Colonic mucosal tissue (rectum - sigmoid junction) was biopsied 4 pieces under colonoscope. ARE binding activity was detected by EMSA and the expression of HO-1 was detected by immunohistochemistry staining methods. 　　RESULTS: The ARE binging activity in UC colonic mucosa is higher than that in the control group, as follows: severe active group gt; mild - moderate active group gt; control group. The HO-1 expression in UC colonic mucosa is higher than that in the control group, as follows: severe active groupgt; mild-moderate active group gt; control group. The change of ARE binging activity and HO-1 expression in UC patients is of positive correlation. 　　CONCLUSION: With the further enhancement of the UC disease severity, the ARE binging activity in UC patients colonic mucosa become stronger. The HO-1 expression detected by immunohistochemistry is also increasing. The relationship of ARE and HO-1 is of positiv