分析化学仪器分析气象色谱法实验.pptVIP

Determination of Alcohol in Tincture by Gas Chromatography Tincture Chromatographic Conditions Internal Standard Method * Objectives Equipments and chemicals Shimadzu GC-14B gas chromatographic system; microsyringe (10 μl); suction pipets (5ml,10 ml); volumetric flask (100 ml) 1.Grasp quantitative method for internal standardization comparison. 2.Grasp the gas chromatographic method for determination of alcohol in tincture. 3.Grasp the operations for gas chromatographic system. Equipments Chemicals Absolute alcohol (A,R); Anhydrous propyl alcohol (internal standard); Tincture sample. A tincture is typically an alcoholic extract of plant or animal material or solution of such or of a low volatility substance (such as iodine and mercurochrome). To qualify as an alcoholic tincture, the extract should have an ethanol percentage of at least 25–60% (50–120 US proof).[citation needed] Sometimes an alcohol concentration as high as 90% is used in such a tincture. In herbal medicine, alcoholic tinctures are made with various ethanol concentrations, 25% being the most common. The main components of GC system Packed column;2m×3mm;80℃ column 10% PEG-20M(polyethylene glycol-20M,polar stationary phase) Stationary phase carrier gas N2, 0.1mPa Injector port 150℃ support 102 white support detector flame ionization detector;200℃ H2 0.3mPa Air 0.2mPa The chromatographic column 1. Add the same amount of an internal standard substance into both the standard solution and sample solution. 2.The concentration of solution is in proportion to the ratio of area of internal standard peak and sample peak in the same solution. 3.The internal standard substance used in this method can minimize the influence of conditions on determination. Internal standard substance: propanol 1.Preparation of solutions Standard solution = absolute alcohol + propanol + distilled water (100ml) (5.00ml) (5.00ml)