反义寡核苷酸阳离子脂质体的制备及癌细胞体外转染效率研究.pdfVIP

反义寡核苷酸阳离子脂质体的制备及癌细胞体外转染效率 刘洋李坤梅芋张振中 (鄢j|IJ{大学药学院，溯南郑州450001) 镰薹：目的-制蔷载反义寡援替酸豹阳离予月籍瓶体并考察斡癌细胞的抑制情醒及体外转祭效率。方法；以磷脂一 十八胺一融陶醉为类聪成分，薄睽超声一吸附一冷猿千燎法制备稳定的载J辽义=家拔背酸阳离予脂疆体。激光粒艘仅 礁定冷冻午操箭麟脂质体zeta魄俄及糍授，透射电镜观察其形态，葡聚籀凝睦枝分离来包封的反义彝桉替酸。紫 外法渊窀礞千德后的毂翁举。Mw法榆测反义寡援臀酸对卵巢瘸细胞WCF-7的抻涮。荧光髭微镜测定不同时湖细 胞摄取荧光标记m的II青况。结祭；辩藻镢筠甘舔聪及拷氯醺为较好的冻千保护剂。制得的阳离予脂质体前体雅正 矬崧，脯璇体的栽药帑链溜瞒为83．21％。豚质体介导的5’邛I健炎光标jj已的asO[搿挠卵巢斑靴F纲脆中的平 均睾睾染搴2h、4b、8h分别为18％，2B％，38％，捧且细胞生长明篡被抻劁，与对照魍比较，其谢显蔷性惹异 (p《O．05)。结论；簿藻糖作为球午保护荆，薄膜翘声一吸附一冷j竭f；下燎法可成功赫焉备反义j落梭替酸潮璃乎脂璇体， 稳定性-犬丈改善。诙豚质体分辱的端靛醣asOON能够有效抑制卵巢癌★ICF-7期胞生长，体井转缝效率较高。 必键词：药举；反义塞拨咎酸；阳离予脂瓶体；转染放举 ofAntisense Preparation Cationic anditsTransfection Liposomes Efficiency LIKun，LIU Qian，ZHANG Yang，MEI zhenzhong of 45000 (CollegePharmacy,ZhengzhouUniversity,Zhengzhou1) To stableantisense cationie and Abstract：0bjectiveprepare liposomes oligodeoxynucleofides*loaded evaluatethetransfection ofasODNtoMCF cells．MethodsasODN·loaded efficiency oophomma was cationic formulation athin method liposomal preparedby whichis andCanovercomecmcial simple pharmaceutical formulation was transmissionelectron duringstorage，Tlle by liposomal morphologyinvestigated sizeandsurface ofthe Were determinedlaser Microscope．The charge liposomes by particle