一株高效纤维素降解菌株的分离鉴定及其酶学性质.pdfVIP

微生物学通报 APR 20, 2010, 37(4): 524528 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 一株高效纤维素降解菌株的分离鉴定 及其酶学性质 钱林 郑巧利 付瑾 王慧萍 郑春丽 柳建设* (东华大学环境科学与工程学院 上海 201620) 摘 要: 从黄浦江淀山湖的水底沉积物中筛选分离得到一株产纤维素酶的菌株。经细菌形态观察, 生理生化实验并结合 16S rRNA 序列分析, 鉴定该菌为蜡状芽孢杆菌 (Bacillus cereus), 同时发现其 在系统发育树中处于一个独立的分支, 推测该菌为 Bacillus 属中一个新的亚种。对该菌株产酶及酶 活特性进行了初步研究, 发现纤维素酶的产生与细菌的生长密切相关。该菌株在37°C, pH 7.0 的条 件下, 发酵66 h 后纤维素酶活达到最高值4.58 U/mL 。 关键词: 纤维素酶, 降解, 16S rRNA, 蜡状芽孢杆菌 Isolation and Identification of a Cellulose Degrading Bacterium and Its Cellulase Characterization QIAN Lin ZHENG Qiao-Li FU Jin WANG Hui-Ping * ZHENG Chun-Li LIU Jian-She (School of Environmental Science and Engineering, Donghua University, Shanghai 201620, China) Abstract: A cellulose degrading strain DSH was isolated from the sediment of Dianshan Lake, Huangpu River, Shanghai. Basing on morphology, biochemical and physiological characterization, DSH was charac- terized as Bacillus cereus . A phylogenetic tree was constructed with the published 16S rRNA sequences of relative bacteria species. In the phylogenetic tree, the DSH strain is in a new branch which has the closest relative to Bacillus cereus strain JBE0004 with 99.78% sequence similarity. The generation of cellulose was closely correlated to its growth rate. The highest cellulose activity of DSH strain was 4.58 U/mL when grown at 37°C, pH 7.0 for 66 h. Keywords: C