薯蓣皂苷元氨基酸衍生物的制备及其抗肿瘤作用研究.pdfVIP

6 3 Vol. 6 No. 3 2015 3 Journal of Food Safety and Quality Mar. , 2015 何忠梅, 徐冰芳, 李 阔, 杨 鹤, 祝洪艳, 郜玉钢, 赵 岩, 张连学* (, 130018) 摘 要: 目的 , , 方法 , , (MTT) , Caco-2 结果 4 , , , Caco-2 MGC-709 SPC-A-1 SH-SY5Y 4 , 3 Caco-2 , S , G2/M 结论 , Caco-2 关键词: ; ; ; Research on the preparation of amino acid derivatives of diosgenin and its anti-tumor effect HE Zhong-Mei, XU Bing-Fang, LI Kuo, YANG He, ZHU Hong-Yan, GAO Yu-Gang, * ZHAO Yan, ZHANG Lian-Xue (College of Chinese Medicine Materials, Jilin Agricultural University , Changchun 130116130018, China) ABSTRACT: Objective To synthesize the amino acid derivates of diosgenin, investigate the effect of derivates on inhibition of human tumor cell growth and study its antitumor mechanism preliminarily. Methods The derivates of amino acid from diosgenin were prepared by esterification reaction. The chemical structure of derivatives was determined by nuclear magnetic resonance (NMR) spectroscopy. The inhibitive effect of cell growth of derivatives was measured by MTT assay. Flow cytometic analysis was used to detect cell phase distribution of Caco-2 cell. Results Four amino acid