Development of an EL ISA for the α-Cyano Pyrethroids Multiresidue in Tai Lake Water精选.pdf

J. Agric. Food Chem. 2009, 57, 3033–3039 3033 Development of an Enzyme-Linked Immunosorbent Assay for the r-Cyano Pyrethroids Multiresidue in Tai Lake Water X. L. HAO,† H. KUANG,‡ Y. L. LI,† Y. YUAN,† C. F. PENG,† W. CHEN,† L. B. WANG,*,† AND C. L. XU*,† School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu, 214000, China, and Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China A general and broad class selective competitive enzyme-linked immunosorbent assay (ELISA) for the detection of the R-cyano pyrethroids was developed. One class specific hapten, the 5-(3- benzylphenyl)-5-cyanopentanoic acid (CPBA-BE), was conjugated with BSA as an immunogen. Polyclonal antibodies, PY-antibodies, were generated. The assay with the most selectivity for the family R-cyano pyrethroids such as cyphenothrin was further optimized and tested for tolerance to cosolvent, pH, and ionic strength changes. The IC50 values of the optimized immunoassay were 4.58 µ g L-1 for cyphenothrin, 5.62 µ g L-1 for fenpropathrin, 7.08 µ g L-1 for deltamethrin, 10.72 µ g L-1 for cypermethrin, 19.95 µ g L-1 for flucythrinate, and 28.18 µ g L-1 for esfenvalerate. The cross- reactivities of cyphenothrin, fenpropathrin, deltamethrin, cypermethrin, flucythrinate, and esfenvalerate were 100%, 81.49%, 64.68%,