叶片尺度光合荧光实验设计PPT.ppt

湿度响应曲线解释的问题 设施栽培中需要精细化管理，使大棚中保持适宜的温湿度，从而提高杨梅净光合速率。 ECOTEK 湿度响应曲线测量方法 为了检验其关系，实验控制光强 1500μmolm-2·s-1，CO2 浓度375 μmolmol-1，温度32℃ ，采取50% 、55% 、60% 、65% 、70% 、75% 、80% 共7 个湿度梯度测定杨梅净光合速率。 ECOTEK 湿度响应曲线实验小结 实验仪器：Li-6400（XT）6400-40/6400-02B/6400-18 采样：略 时间：略 重复数：略 光适应：30分钟 温度：略 二氧化碳浓度：控制 湿度:设置湿度梯度 ECOTEK 二氧化碳响应曲线 (Masabumi et al. 2012) ECOTEK 二氧化碳响应曲线解释的问题 生在在高二氧化碳浓度下的植物Rubisco羧化能力下降 Plants grown under elevated [CO2] exhibited photosynthetic down regulation, indicated by a decrease in the carboxylation capacity of Rubisco. 白桦树幼苗最大羧化速率和RuBP再生速率下降 White birch seedlings grown at elevated [CO2] under limited N supply showed photosynthetic down regulation, indicated by decreases in the maximum rates of RuBP carboxylation (Vcmax) and RuBP regeneration(Jmax ) (Masabumi et al. 2012) ECOTEK 二氧化碳响应曲线测量方法 The maximum rates of RuBP carboxylation (Vcmax)and RuBP regeneration were derived from photosynthetic responses to various Ci at a leaf temperature of 25℃ and saturating photon flux density (PFD) of 1000μmol m?2s?1, which was provided by a red/blue light emitting diode (LED) array （6400-40;Licor）with blue light comprising 10% of total PFD . (Masabumi et al. 2012) ECOTEK 二氧化碳响应曲线实验小结 实验仪器：Li-6400（XT）6400-40 采样：生长50天完全展开的叶片 时间：依据二氧化碳浓度梯度个数而定 重复数：n=8 光：适应30分钟 温度：适应30分钟 二氧化碳浓度：梯度 湿度:不控 ECOTEK 荧光-光响应曲线 (Mitsutoshi et al. 2012) ECOTEK 荧光-光响应曲线解释的问题 The shade leaves of the Japanese oak grown within a crown were suggested to adjust their N investment to maintain higher photosynthetic capacities compared with those required to maximize the net carbon gain, which may facilitate the dissipation of the excessive light energy of sunfleck