生理学11鼓楼医院朱大龙会议内分泌与代谢.ppt

Estrogen activates hypothalamic STAT3 while promoting satiety Qian Gao The Medical School of Nanjing university Ovariectomy (OVX) induces food intake and adiposity. E2 replacement diminishes food intake and adiposity of OVX animals. What is the relationship between synaptic changes and energy homeostasis? * P0.05 compared to all groups Leptin signaling Diabetes Summary: E2 treatment increases excitatory synapses on POMC neurons and enhances POMC tone E2 decreases food intake and body weight in WTs, ob/ob, db/db mice E2 effect is independent of leptin E2 effect seems to be mediated by Stat3. Yale University The Rockefeller Sabrina Diano UniversityYongzhan Nie Jeffrey Friedman Yan Rao Gabor MezeiXiao-Bing Gao Gerald Shulman Support Tamas Horvath NIH-NIDDK Mount SinaiCharles Mobbs   Columbia University  Dominique Toran-Allerand ACKNOWLEDGEMENT Males Females 4 week of treatment Nuclear magnetic resonance imaging 1 day of treatment Diet-induced thermogenesis Estrogen mimics leptin’s effect on the rewiring of POMC cells independently of leptin receptor-mediated signaling Leptin signaling E2, Peptide Hormone, Nutrition factors and Environmental inputs Transmitters POMC, NPY Synaptic plasticity ?? Behavioral adaptations Cellular adaptations Food intake Energy expenditure ?? P-Stat3 was induced by estrogen in mouse hypothalamus Estrogen: 10ug/mouse in 500ul saline. b-actin STAT3 pSTAT3 0 15 30 60 90 120 150 E2 induces p-Src, but not p-Jak1 p-Jak2 in mouse hypothalamus * Hela cells as positive control of p-Src 0 30 60 0 60 0 60 P hypoth cortex liver hela* P-Src b-actin P-JAK1 P-JAK2 Src Leptin signaling deficiencies * Workshop on steroid Hormones and Brain Function, Key Largo, Florida, April 8-12, 2008 Estrogen activates hypothalamic STAT3 while promoting satiety Qian Gao The Medical School of Nanjing university Workshop on steroid Hormones a