肿瘤干细胞 临床一对 郑楠薪 刘泓毅 刘洋.pptVIP

The Discussion of Cancer Stem Cells（CSC） 临床一队 郑楠薪 刘泓毅 刘洋 FUNDAMENTAL QUESTIONS What makes the 200 cell types in our body remember their identity? What’s the relationship between stem cells and CSC? Do we have any ways in horizon to conquer cancer? The Definition of Stem Cells Stem cells are defined as cells that have the ability to perpetuate themselves through self-renewal and to generate mature cells of a particular tissue through differentiation. In most tissues, stem cells are rare. Tannishtha Reya, Sean J. Morrison, Michael F. Clarke and Irving L. Weissman. Nature 414, 105-111，2001 Development of haematopoietic stem cells CLP: common lymphoid progenitors CMP: common myeloid progenitors GMP: granulocyte macrophage precursors MEP: megakaryocyte erythrocyte precursor MkP: megakaryocyte precursors ErP: erythrocyte precursor Hepatic cancer brain cancer pulmonary cancer colonic cancer breast cancer leukemia prostatic cancer skin cancer Hepatic cancer The Occurrence And Progression of Tumor Concept of cancer stem cells Cancer stem cells have been defined as ‘a cell within a tumor that possess (sic) the capacity to self-renew and to cause the heterogeneous lineages of cancer cells that comprise the tumor’ (Clarke et al, 2006). Property Definition Assay Self-renewal The ability to undergo symmetric division Re-plating assays And thereby indefinitely replenish itself Serial transplantation Differentiation capacity The ability to undergo asymmetric division and thereby recapitulate all tumor cell types Differentiation Transplantation Tumor initiation Metastasis Relapse The ability to propagate tumor when transplanted into the proper environment The ability of resistance to different therapies and the ability to relapse Sphere formation Invasion assays Transplantation Chemo/radio-resistance assays Cancer Stem Cell Properties Evidence for Cancer Stem Cells 1. 1/10000 to 1/100 cancer cells from m