油茶内生细菌Y13诱导油茶植株对炭疽病系统抗性研究.PDFVIP

油茶内生细菌Y13 诱导油茶植株对炭疽病系 统抗性研究 周国英，宋光桃，布婷婷，李石磊，赵莹，王慧敏 （中南林业科技大学 经济林培育与保护教育部重点实验室，湖南 长沙 410004） 摘 要：油茶内生细菌Y13 发酵液可以诱导油茶叶片对炭疽病的系统抗性。Y13 诱导处理后，植株叶内苯 丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)和超氧化物歧化酶(SOD)活性明显增强。Y13 单 独诱导、炭疽病菌单独接种和同时Y13 和炭疽病处理诱导后各种防御酶活性较清水对照均有所提高，同时 Y13 和炭疽病处理组诱导处理第3 d PAL 活性达到最大，为清水对照的2.9 倍，只接种Y13 及炭疽病处理 组PAL 活性于第5 d 达到高峰，分别是对照组的2.1 和2.8 倍；POD 和SOD 诱导后第5 d 活性最高，分别 比对照增加了15.5-29.5 及8.5-20.5 个酶活性单位，11 d 后仍比对照高；PPO 诱导后3 d 达活性高峰，7d - 后迅速下降，后趋于正常水平。活性氧(O )产生速率诱导后1 d 最大，比对照增加80.6%，随后下降，10d 2 左右接近对照，Y13 单独诱导和同时Y13 和炭疽病菌诱导均使MDA 含量在第 1d 时迅速下降，第3d 左右比 对照下降了36%。 关键词：内生细菌Y13；油茶炭疽病；防御酶系；诱导抗性 Induction effects of Endophytic BacteriaY13 on the Resistance Associated Enzymes Activities in Camellia ZHOU Guo-ying ，SONG Guangtao, BU Ting-ting ，ZHAO Ying ，LI Shi-lei ，DENG Xiao-jun (Nonwood Forest Cultivation and Protection Key Laboratory of the Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan 410004) Abstract: Systemic resistance in camellia leaves against anthracnose could be induced by endophytic bacteria Y13 strains in the camellia. After induction treatment of Y13, the activities of phenylalanine ammonia lyase (PAL), peroxidase (POD), polyphenol oxidase (PPO) and superoxide dismutase (SOD) in the plant leaves inereased obviously. Compared with control, the defense enzyme activity Vaccination Y13, anthrax bacteria and simultaneously inoculation Y13 and anthrax after induction treatment is improved, and the PAL activity of vaccination Y13 and anthrax reached their maximum 3 days after the treatment, and showed 2.9 fold higher more than the water control treatment. When only anthrax vaccine or Y13, the PAL activity reached a peak in 5 days, 1.7 and 2.3 fold higher more than the water control tre