LC-MSMS同时测定大鼠血浆中5种银杏酸浓度及其药动学研.PDFVIP

LC-MS/MS 同时测定大鼠血浆中 5 种银杏酸浓度及其药动学研究  * 李力，李贺，黄越，王国付，韩奇，刘炜(浙江医院药剂科，浙江省老年医学重点实验室，杭州 310013) 摘要：目的 建立LC-MS/MS 同时测定大鼠血浆中5 种银杏酸的浓度，并进行药动学研究。方法 大鼠血浆样品用乙酸 乙酯萃取后，以水杨酸为内标，选用ZORBAX Eclipse XDB C18(2.1 mm×100 mm ，3.5 μm) 色谱柱，以乙腈-水(均含0.1% 甲酸)为流动相梯度洗脱，梯度流速恒定为0.25 mL·min1 ，柱温为30 ℃，进样量为10 μL ，总分析时间为5.0 min ，采用 电喷雾离子化源，负离子方式，多反应监测(MRM)扫描方式进行监测。结果 血浆中 5 种银杏酸浓度线性范围为 2.0~ 1 000 μg·L1(r0.99) ，定量下限为2.0 μg·L1；质控样品准确度为92.8%~108.5%；日内、日间RSD 均15%；提取回收率 为73.3%~86.2% 。结论 该方法快速、灵敏、准确，专属性强，重复性好，适用于同时测定大鼠血浆中5 种银杏酸浓度。 关键词：银杏酸；液相色谱串联质谱法；血药浓度；药动学 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2017)12-1658-05 DOI: 10.13748/ki.issn1007-7693.2017.12.003 引用本文：李力, 李贺, 黄越, 等. LC-MS/MS 同时测定大鼠血浆中 5 种银杏酸浓度及其药动学研究[J]. 中国现代应用药学, 2017, 34(12): 1658-1662. Simultaneous Determination of Five Ginkgolic Acids in Rat Plasma by LC-MS/MS and the Application in Pharmacokinetics Study * LI Li, LI He, HUANG Yue, WANG Guofu, HAN Qi, LIU Wei (Department of Pharmacy, Zhejiang Hospital, Zhejiang Provincial Key Laboratory of Geriatrics, Hangzhou 310013, China) ABSTRACT: OBJECTIVE To develop an LC-MS/MS method for simultaneous determination of five ginkgolic acids in rat plasma and applied to the pharmacokinetics study. METHODS The plasma samples were processed by ethyl acetate extraction, with salicylic acid as an internal standard. The separation was achieved on ZORBAX Eclipse XDB C18 column (2.1 mm×100 mm, 3.5 μm) and eluted with linear gradient using acetonitrile and water (both of containing 0.1% formic acid) at the flow rate of 0.25 mL·min1, the injection volume was 10 μL and the column temperature was 30 ℃. The total time o