异土木香内酯降低金黄色葡萄球菌α-溶血素的表达-中国农学通报.PDFVIP

中国农学通报 2011,27(32):30-33 Chinese Agricultural Science Bulletin 异土木香内酯降低金黄色葡萄球菌α-溶血素的表达 1,2 1 1 吴金梅 ，邱家章 ，邓旭明 1 2 （吉林大学畜牧兽医学院，长春130062；郑州牧业工程高等专科学校，郑州450002 ） 摘 要：为了考察异土木香内酯对金黄色葡萄球菌的抗菌活性，并研究其对金葡菌α-溶血素表达的影响， 应用肉汤微量稀释法测定异土木香内酯对金葡菌标准株的最低抑菌浓度，采用溶血试验、Western-blot 分析和荧光定量PCR 分析测定异土木香内酯对金葡菌α-溶血素表达的影响。结果表明，异土木香内酯 在2048 μg/mL 仍然不能抑制金葡菌的生长，然而低浓度（1~8 μg/mL ）的异土木香内酯能够剂量依赖性 地降低金葡菌α-溶血素的表达。该研究提示异土木香内酯可作为以毒力因子为靶标的抗感染药物开发 的先导结构。 关键词：金黄色葡萄球菌；α-溶血素；异土木香内酯 中图分类号：S859.7 文献标志码：A 论文编号：2011-1628 Isoalantolactone Reduces α-toxin Production by Staphylococcus aureus 1,2 1 1 Wu Jinmei , Qiu Jiazhang , Deng Xuming 1 ( College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062; 2Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450002) Abstract: In the study, we aimed to investigate the antimicrobial activity of isoalantolactone against Staphylococcus aureus, and further assess the influence of isoalantolactone on the production of α-toxin by S. aureus. The minimal inhibitory concentrations (MICs) were determined by a broth micro-dilution method. The effects of isoalantolactone on α-toxin production were assessed by hemolysis, western blot, and real-time RT-PCR assays. The MICs of isoalantolactone against S. aureus strains were greater than 2048 μg/mL. However, low concentrations of isoalantolactone (1-8 μg/mL) could subs