Rho激酶抑制剂与糖尿病心肌病PPT.ppt

Immunocytochemical staining for type-I procollagen. Magnification is ×400. 结 果 Accumulation of type-I collagen in media was determined by ELISA. The data are means ± SD. n=6; ?P0.01 vs NG group. *P0.05, **P0.01 vs HG group. 结 果 结 果 The phosphorylation of MYPT1 was measured by western blot analyses. n=3; ?P0.01 vs NG group. **P0.01 vs. HG group. 结 果 JNK activity and c-jun protein expression were measured by Western blot analyses. n=3; ?P0.01 vs NG group. **P0.01 vs HG group. 结 果 The protein expression of TGFβ1 and the phosphorylation of Smad2/3 was measured by Western blot analyses. n=3; ?P0.01 vs NG group. **P0.01 vs HG group. 结 论 结 论 高糖激活了RhoA/ROCK通路，介导了心肌成纤维细胞增殖和胶原合成。ROCK特异性抑制剂法舒地尔有效地抑制了高糖诱导的成纤维细胞增殖和胶原合成。 高糖环境中，JNK和Smad2/3是ROCK的下游信号分子，RhoA/ROCK通路介导的胞质信号部分地通过JNK和Smad2/3转入核内，从而调控着相关的基因表达。 第二部分 RhoA/ROCK通路在2型糖尿病大鼠心肌纤维化中的作用及法舒地尔对心肌的保护效应 在体研究 2型糖尿病大鼠 普通饲料喂养 10mg/kg i.p. Fasudil治疗大鼠 糖尿病大鼠 对照组大鼠 对照组大鼠 SD大鼠 高脂饲料喂养 6w末STZ 30mg/kg i.p. 10w末血糖嵌夹 24w末结束试验 实验设计 结 果 结 果 结 果 Masson staining, Magnification, ×400 结 果 TEM, Magnification, ×6000 结 果 SABC staining for type-I collagen (A) and type-III collagen (B) Magnification, ×400 结 果 The mRNA expression of type-I procollagen, type-Ш procollagen was evaluated by RT-PCR. n=6; P0.01. 结 果 The mRNA expression of ROCK1, JNK and TGFβ1 was evaluated by RT-PCR. n=6; 结 果 The phosphorylation of MYPT1 and JNK was measured by Western blot analyses. n=6; P0.01. 结 果 The protein levels of c-jun and TGFβ1, as well as p-Smad, were evaluated by Western blot analyses. n=6; P0.01. 结 论 糖尿病大鼠发生了心肌纤维化，出现了心脏收缩和舒张功能障碍。 RhoA/ROCK通路在2型糖尿病大鼠心肌纤维化中起了重要作用，这可能与它激活JNK和TGFβ1/Smad通路密切相关。 法舒地尔有效地抑制了糖尿病心肌纤维化，它对心肌的这种结构性益处不依赖于血糖和血压的改善。 * * P o w e r B a r 中国专业PPT设计交流论坛 * Company Logo Rho激酶抑制剂 与糖尿病心肌病 河北医科大学第二医院心内科 李拥军 Rho激酶的结构和功能 Rho激酶抑制剂的药理作用 3. Rho激酶抑制剂与心血管疾病 4.