靶向PTTG1的siRNA对肝癌SMMC-7721细胞迁移和侵袭的作用李.DOCVIP

靶向PTTG1的siRNA对肝癌SMMC-7721细胞迁移和侵袭的作用 李中堂，吴忠均 （重庆医科大学附属第一医院：肝胆外科,重庆 400016） 【摘 要】 目的:首先是检测垂体肿瘤转化基因1（Pituitary Tumor Transforming Gene 1,PTTG1）在肝癌(HepG2,SMMC-7721)细胞和正常细胞(LO2)中的表达差异。然后通过转染小干扰 RNA(siRNA)抑制 PTTG1，探寻其在肝癌 SMMC-7721细胞中的迁移和侵袭作用。 方法:在 HepG2,SMMC-7721及 LO2细胞中通过 RT-PCR和 Western Blot检测 PTTG1的 mRNA和蛋白质的表达。然后将构建的 PTTG1 siRNA转染到 SMMC-7721细胞中,再用 Transwell小室分析探讨PTTG1对SMMC-7721细胞迁移和侵袭的作用。 结果:肝癌 HepG2细胞和 SMMC-7721细胞中，PTTG1的mRNA和蛋白质的水平比 LO2细胞明显升高，且 SMMC-7721细胞升高更明显。siRNA干扰 PTTG1基因后, SMMC-7721细胞迁移和侵袭明显受抑,差异均具有统计学意义(P 0. 05)。　结论:靶向 PTTG1的 siRNA下调 PTTG1表达后可抑制 SMMC-7721细胞的迁移和侵袭。所以 PTTG1在肝癌的发生发展过程中可能作为一癌基因促进肝癌的转移。 【关键词】肝癌;PTTG1基因;迁移；侵袭；SMMC-7721细胞 Effect of siRNA targeting PTTG1 gene on migration and invasion of human hepatoma SMMC-7721 cells Li Zongtang,Wu Zhongjun (Department of Hepatobiliary surgery First Affiliated Hospital, Chongqing Medical University, Chongqing 400016) 【Abstract】Objective The aim of the present study is to analyze the different expression of Pituitary Tumor Transforming Gene 1 (PTTG1) between hepatoma(HepG2,SMMC-7721) cells and normal(LO2) cells, and then to explore the effect on migration and invasion of SMMC-7721 cells after transfected small interfering RNA(siRNA) inhibited PTTG1. Methods: PTTG1 mRNA and protein expression were determined by RT-PCR and Western blot among HepG2, SMMC-7721 and LO2 cells. In addition, siRNA of PTTG1 were constructed and transfected into SMMC-7721 cells. The effect of PTTG1 gene on migration and invasion of SMMC-7721 cells was detected by transwell assay, respectively. Results: Our results showed that (the expression levels of PTTG1 mRNA and protein in HepG2 and SMMC-7721 cells significantly higher than LO2 cells , especially in SMMC-7721 cells. After knock-down PTTG1 by siRNA, the migration and invasion of SMMC-7721 cells was significantly inhibited. There were statistically significant differences﹙P＜0.05﹚. Conclusion: Knocked-down PTTG1 by siRNA could effectively inhibit migration and invasion of SMMC-7721 cells.PTT