过氧化氢酶和Bcl-2在实验性精索静脉曲张大鼠睾丸中的变化关系.docVIP

精品论文 参考文献 过氧化氢酶和Bcl-2在实验性精索静脉曲张大鼠睾丸中的变化关系 王建伟1 贾月峰2(通讯作者) 张勇2 杨孝永3 徐锦2 贺萍丽2 (1东营市广饶县人民医院 山东东营 257300) (2青岛大学医学院附属医院 山东青岛 266003) (3临沂市兰山区义堂中心卫生院 山东临沂 276013) 【摘要】目的 建立大鼠实验性精索静脉曲张（experimental varicocele EV）的模型，测量睾丸中过氧化氢酶（catalase CAT）活性和Bcl-2的表达，并分析两者的变化关系。方法 将30只雄性青春期Wistar大鼠随机分为EV8周组和12周组（各9只）和相应的假手术对照组2组（各6只），通过部分结扎左肾静脉建立大鼠EV模型，分别于术后8周、12周处死动物，测左侧精索静脉直径，用比色法测CAT活力，免疫组化法测Bcl-2的表达。结果 成功建立了EV模型，与相应的对照组相比左侧精索静脉直径明显增大（Plt;0.01）。EV组双侧睾丸的CAT活性显著低于相应的对照组（Plt;0.01），左侧睾丸比右侧睾丸更低，但无明显统计学意义（P＞0.05）。EV组双侧睾丸间质细胞中Bcl-2的染色指数与相应的对照组相比均显著降低（Plt;0.01），左侧睾丸染色指数比右侧睾丸下降更明显（Plt;0.01），EV12周组与EV8周组相比，EV12周组染色指数更低（Plt;0.05）。CAT活性与Bcl- 2的染色指数在0.01水平有显著相关性。结论 我们对EV模型做了观察和研究，认为过氧化氢酶通过抑制氧化应激可能影响Bcl-2并抑制细胞凋亡。 【关键词】精索静脉曲张 氧化应激 凋亡 过氧化氢酶 Bcl-2 【中图分类号】R965 【文献标识码】A 【文章编号】2095-1752（2013）27-0031-02 The Variation of CAT and Bcl-2 in the Testes of Experimental Varicocele Rats 【Abstract】 Objective To establish experimental varicocele (EV) model in rats to measure the catalase (CAT) activity and the Bcl-2 expression in the testes, and analyze the variation of CAT and Bcl-2.Methods The varicocele model was created by partial ligation of the left renal vein in adolescent male Wistar rats.A total of 30 male adolescent Wistar rats were randomly divided into 8-week EV group, 12-week EV group and the corresponding sham-operated control group. The rats were killed 8 weeks and 12 weeks after operation, the histology of the testis was studied, the activity of CAT was determined by Colorimetry and the Bcl-2 was determined by Immunohistochemistry. Results The experimental varicocele model was successfully created. Compared with the corresponding control group, the diameter of the left spermatic vein enlarged remarkably(P＜0.001). The CAT activity of the bilateral testes in EV groups was decreased significantly compared with that in the corresponding control groups (Plt;0.01) and the left sides were lower than the right sides, but had