hplc同时测定盆炎净胶囊中3种有效成分含量.docVIP

HPLC同时测定盆炎净胶囊中3种有效成分含量 　　摘要：目的 建立HPLC同时测定盆炎净胶囊中3种有效成分芍药苷、原儿茶酸和绿原酸的含量分析方法。方法 采用SHIMADZU VP-ODS-C18色谱柱（250 mm×4.6 mm，5 ?m），流动相为乙腈-0.15%磷酸溶液梯度洗脱，检测波长231、255、326 nm，流速1.0 mL/min，柱温30 ℃，进样量20 ?L。结果 在上述色谱条件下，芍药苷、原儿茶酸、绿原酸之间分离度良好。芍药苷Y=1546.412 8X+127.075 6（r=0.999 9），线性范围150.054～1254.50 ng；原儿茶酸Y=2925.846 8X+2204.107 6（r=0.999 8），线性范围162.33～1352.75 ng；绿原酸Y=893.904 5X-261.731 5（r=0.999 4），线性范围11.43～95.25 ng。3种成分的平均回收率分别为97.60%、102.09%、98.52%。结论 该方法操作简单、方便、准确，可为盆炎净胶囊的质量控制提供借鉴。 　　关键词：盆炎净?z囊；芍药苷；原儿茶酸；绿原酸；高效液相色谱法 　　DOI：10.3969/j.issn.1005-5304.2017.05.019 　　中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2017）05-0082-04 　　Simultaneous Contents Determination of Three Active Ingredients in Penyanjing Capsules by HPLC YUAN Zhi-ying1， YANG Yan-tao1， LI Rong-dong1， LI Zhe1， OUYANG Ji-de2 （1. Hunan University of Chinese Medicine， Changsha 410208， China； 2. Chenzhou Institute for Food and Drug Control， Chenzhou 423000， China） 　　Abstract： Objective To establish an HPLC method for simultaneous contents determination of paeoniflorin， protocatechuic acid and chlorogenic acid in Penyanjing Capsule. Methods The chromatographic separation was performed on a SHIMADZU VP-ODS-C18 column （4.6 mm × 250 mm， 5 ?m）； the column temperature was maintained at 30 ℃； A gradient elution of acetonitrile-0.15% phosphoric acid aqueous solution was adopted at the flow rate of 1.0 mL/min； The UV detection wavelengths were at 231， 255， and 326 nm； The injection volume was 20 ?L. Results Paeoniflorin， protocatechuic acid and chlorogenic acid could be separated efficiently with this condition. The regression equation was Y=1546.412 8X+127.075 6 （r=0.999 9）， Y=2925.846 8X+2204.107 6 （r=0.999 8）， Y=893.904 5X-261.731 5 （r=0.999 4）， respectively. Paeoniflorin， protocatechuic acid and chlorogenic acid were linear in the range of 150.054?C1254.50 ng， 162.33?C1352.75 ng， 11.43?C95.25 ng， and the average recoveries were 97.60%， 102.09% and 98.52%. Conclusion The method is simple， convenient and accurate， which can be used to t