nlrp3与急性心肌梗死患者预后的研究.docVIP

NLRP3与急性心肌梗死患者预后的研究 　　【摘要】 目的 探讨Nod样受体蛋白3（NLRP3）对急性心肌梗死患者预后的预测价值， 为NLRP3的临床应用提供依据。方法 155例因急性心肌梗死入院治疗患者为研究对象， 收集临床资料， 分离外周血单个核细胞， 以免疫印迹法（Western blotting）定量测定NLRP3水平， 测定B型钠尿肽前体（NT-proBNP）及左室射血分数（LVEF）。根据1年随访结果， 将研究对象分为无不良心血管事件（MACE）组（115例）及MACE组（42例）， 观察患者预后与NLRP3表达水平的关系。结果 两组患者的年龄、性别、体质量指数（BMI）、吸烟、低密度脂蛋白胆固醇（LDL-C）、阿司匹林使用率、氯吡格雷使用率、他汀使用率比?^， 差异无统计学意义（P 　　【关键词】 Nod样受体蛋白；急性心肌梗死；预后 　　DOI：10.14163/j.cnki.11-5547/r.2017.04.002 　　【Abstract】 Objective To explore the predictive value of pyrin domain-containing 3 （NLRP3） on prognosis of patients with acute myocardial infarction， and to provide basis for clinical application of NLRP3. Methods A total of 155 hospitalized patients due to acute myocardial infarction as study subjects， and their clinical data were collected. Peripheral blood mononuclear cells were separated， and immunoblotting method （Western blotting） was used to quantitative determination of NLRP3 level， as well as detection of N-terminal pro-B type natriuretic peptide （NT-proBNP） and left ventricular ejection fraction （LVEF）. Study subjects were divided by 1 year follow-up outcomes into non major adverse cardiac events （MACE） group （115 cases） and MACE group （42 cases）. Correlation of prognosis and NLRP3 express level was observed. Results There were no statistically significant difference in age， gender， body mass index （BMI）， smoking， low density lipoprotein cholesterol （LDL-C）， utilization rate of aspirin， clopidogrel and statin between two groups. Compared with non MACE group， MACE group had significantly increased expression of NLRP3 in peripheral blood mononuclear cells， as well as significant increase of NT-proBNP levels significantly， lower LVEF score， lower utilization rate of angiotensin converting enzyme inhibitor/angiotensin receptor antagonist （ACEI/ARB） and beta blockers， lower proportion of patients with hypertension history， and higher proportion of patients with diabetes history. Multiple factors Logistic regression analysis showed that NLRP